Flavonoid Effect on Activation and Stimulation of Thrombocytes, hsCRP and Lipid Profile

NCT ID: NCT00559663

Last Updated: 2007-11-20

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

NA

Total Enrollment

35 participants

Study Classification

INTERVENTIONAL

Study Start Date

2006-09-30

Study Completion Date

2007-04-30

Brief Summary

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The purpose of this study is to compare the effect of dark chocolate with green tea in healthy subjects on clot formation (platelet activity) cholesterol profile and markers of inflammation in two groups.

The first group will be on a regimen of dark chocolate, and the second group will be on a regimen of green tea. Clinical outcomes will be determined after seven days of each treatment and both groups will undergo both treatments subsequently and serve as their own control.

The hypothesis is that both green tea and dark chocolate will reduce platelet activity and reduce LDL, hsCRP and increase HDL.

Detailed Description

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Flavonoids are polyphenolic compounds ubiquitous in fruits and vegetables. They appear in especially high concentrations in the form of flavanols in green tea as well as in cocoa, and are known to have beneficial antioxidant effects in vitro. Flavonoids have been proposed as a key protective dietary component, reducing the risk of coronary heart disease, including the reduction of elevated blood pressure in persons with hypertension and capable of decreasing LDL.

The aim of this prospective single cross-over study is to assess the effect of green tea and dark chocolate consumption on inhibiting platelet activation, Lipid levels, and hsCRP in healthy volunteers.

This research will be done at Sinai Hospital of Baltimore and will include 35 subjects who will be randomized equally between the two groups.

All blood work will be processed at the Sinai Center for Thrombosis Research. Clinical outcomes will be recorded using a standard case report form.

Conditions

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Healthy

Keywords

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Flavonoid hsCRP HDL LDL platelet reactivity

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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GT

The green tea group is given initially green tea treatment and then after a washout period of four weeks switched to the dark chocolate treatment.

Group Type ACTIVE_COMPARATOR

Green tea

Intervention Type DIETARY_SUPPLEMENT

The green tea group consumed first daily one liter of green tea infusate made of 4 bags left in boiling water during 3 minutes.

After a four week washout-time they were switched over to the dark chocolate treatment, which included consumption of 3.5 ounces 70% dark chocolate.

Blood was drawn before and after each intervention, to check platelet activity with flow cytometry, HDL, LDL and hsCRP Levels

DC

The dark chocolate group is given initially dark chocolate treatment and then after a washout period of four weeks switched to the green tea treatment.

Group Type ACTIVE_COMPARATOR

70% dark chocolate

Intervention Type DIETARY_SUPPLEMENT

The dark chocolate group consumed 3.5 ounces 70% dark chocolate per day during a one week period.

After a four week washout-time they were given the green tea treatment, which included ingestion of one liter green tea infusate (containing 4 green tea bags).

Blood was drawn before and after each intervention, to check platelet activity with flow cytometry, HDL, LDL and hsCRP Levels.

Interventions

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Green tea

The green tea group consumed first daily one liter of green tea infusate made of 4 bags left in boiling water during 3 minutes.

After a four week washout-time they were switched over to the dark chocolate treatment, which included consumption of 3.5 ounces 70% dark chocolate.

Blood was drawn before and after each intervention, to check platelet activity with flow cytometry, HDL, LDL and hsCRP Levels

Intervention Type DIETARY_SUPPLEMENT

70% dark chocolate

The dark chocolate group consumed 3.5 ounces 70% dark chocolate per day during a one week period.

After a four week washout-time they were given the green tea treatment, which included ingestion of one liter green tea infusate (containing 4 green tea bags).

Blood was drawn before and after each intervention, to check platelet activity with flow cytometry, HDL, LDL and hsCRP Levels.

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* non-smokers between the ages of 18-60
* BMI of 19 to 30

Exclusion Criteria

* prior usage of Vitamin C \> 1000 mg, Vitamin E \> 400IU, Beta Carotene \> 1000IU, Vitamin A \> 5000IU, Selenium \> 200mcg, Folic Acid \> 1mg, Aspirin or NSAIDs four weeks prior to study initiation
* regular ingestion of Flavonoid rich food (EG green tea, dark chocolate, grapes, red wine and others) prior and during the study except what was provided by the study protocol
* Alcohol or Caffeine consumption 24 prior to blood draws
* Nursing or pregnant women
* blood donation up to eight weeks prior to study initiation
* subjects with coagulation disorders
* known cardiovascular disease (prior myocardial infarction)
* drug abuse
* Diabetes
Minimum Eligible Age

18 Years

Maximum Eligible Age

55 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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LifeBridge Health

OTHER

Sponsor Role lead

Principal Investigators

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Paul A Gurbel, MD

Role: PRINCIPAL_INVESTIGATOR

Sinai Hospital of Baltimore

Miruais S Hamed, MD

Role: STUDY_DIRECTOR

Department of Medicine, Sinai Hospital, Baltimore

Kevin Bliden, BS

Role: STUDY_CHAIR

Sinai Center for Thrombosis Research

Locations

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Center for Thrombosis Research, Sinai Hospital

Baltimore, Maryland, United States

Site Status

Countries

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United States

Other Identifiers

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1242

Identifier Type: -

Identifier Source: secondary_id

1242

Identifier Type: -

Identifier Source: org_study_id