Genetic Studies Spermatogenic Failure

NCT ID: NCT00548977

Last Updated: 2007-10-25

Basic Information

• Recruitment Status: COMPLETED
• Total Enrollment: 283 participants
• Study Classification: OBSERVATIONAL
• Study Start Date: 2001-01-31
• Study Completion Date: 2005-02-28

Brief Summary

The proposed study is designed to test the following hypotheses:

1. Mouse autosomal or X-linked genes which are exclusively expressed in mouse spermatogonia are also spermatogonia-specific in human.

2. Severe spermatogenic defect, especially hypospermatogenesis or SCOS, is caused by an intrinsic defect in germ line stem cell or speramtogenia.

3. Spermatogonia-specific genes are caudate genes for human spermatogenic defect, especially for hypospermatogenesis or SCOS.

4. For a significant fraction of cases with severe spermatogenic defect, the sterile genes are transmitted via multifactorial inheritance mode.

5. For some cases with severe spermatogenic defect, mutations of spermatogonia- specific genes may be transmitted in the X-linked recessive, autosomal recessive, or autosomal dominant mode.

Detailed Description

Between 2% and 12% of couples worldwide are affected by reduced fertility. Men who have defects in sperm production (spermatogenic defect) account for about half of these cases. In Drosophila and mouse, targeted disruptions of numerous sterility- associated genes have been created. Physiological studies in the Drosophila and in mouse also indicate that spermatogenesis is subjected to complex regulation, and male infertility may result from aberrant regulatory events. In the human being, deletions of the Y chromosome account for only 10% of cases with spermatogenic defect, and etiologies of remaining 90% of cases are still unknown. It is evident that multiple genes are involved in male infertility. For cases with severe spermatogenic defect , testicular histology shows either decreased number of germ cells in all developmental stages (hypospermatogenesis) or complete absence of germ cells (Sertoli cell only syndrome or SCOS). It appears that there is an intrinsic defect which causes depletion of germ-line stem cells (spermatogonia) for cases with hypospermatogenesis or SCOS. Of 25 genes exclusively expressed in mouse spermatogonia, 3 are Y-linked, 10 are X-linked, and only 12 are distributed on autosomes.

Conditions

Oligospermia; Azoospermia; Male Infertility

Keywords

spermatogenesis defect; male infertility; azoospermia; oligozoospermia; Y chromosome markers; estrogen gene

Study Design

• Observational Model Type: CASE_CONTROL
• Study Time Perspective: PROSPECTIVE

Interventions

Drawing blood to study genetic polymorphism

Intervention Type: OTHER

Eligibility Criteria

Inclusion Criteria

• Men with oligozoospermia(<2*10^7/ml) or azoospermia

Exclusion Criteria

• Abnormal karyotypes
• Obvious genital trauma history
• Genital hernia
• Other recognizable causes of male infertility

• Minimum Eligible Age: 14 Years
• Maximum Eligible Age: 60 Years
• Eligible Sex: MALE
• Accepts Healthy Volunteers: Yes

Sponsors

National Cheng-Kung University Hospital

OTHER

Sponsor Role: lead

Principal Investigators

Paolin Kuo, MD

Role: STUDY_CHAIR

Locations

National Cheng-Kung University Hospital

Tainan City, , Taiwan

Countries

Taiwan

Other Identifiers

NCKUH-1

Identifier Type: -

Identifier Source: org_study_id