Hematopoietic Stem Cell Therapy in Chronic Wounds Using a Pressure Sore Model

NCT ID: NCT00535548

Last Updated: 2007-09-26

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

UNKNOWN

Clinical Phase

PHASE1

Study Classification

INTERVENTIONAL

Study Start Date

2007-01-31

Study Completion Date

2007-12-31

Brief Summary

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Aim of the study:

Evaluation of feasibility, safety and potential effects of stem cells on chronic wounds using a pressure sore model.

Clinical relevance:

* Accelerated healing of uncomplicated wounds
* Enhanced healing of complicated (chronic, non-healing) wounds

Study design:

* Prospective controlled phase I/II study
* Cohort of 5 patients in pilot study, then reevaluation

Patients:

\- Para- and tetraplegic patients with sacral pressure sores grade III-IVA according to the classification of Daniel and Seiler

Methods:

1\. First surgical intervention:

* Radical debridement of pressure sore
* Bone marrow harvest from the iliac crest
* Isolation of hematopoietic stem cells, aiming to gain \> 1 mio. CD 34+ cells per patient under GMP conditions

3\. Stem cell therapy (after 2 days)
* Injection of stem cells in suspension (50'000 CD 34+ cells in 100 microliter saline per cm2 of wound surface) on one half of the total wound surface and cell-free saline on the other half as a control

4\. Second surgical intervention (after 3-4 weeks):
* Complete excision of the wound
* Closure of the defect by fasciocutaneous flap

5\. Evaluation of wound healing:
* Clinical
* 3D laser imaging
* Histology
* Growth factor assay

Detailed Description

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Conditions

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Chronic Wounds Pressure Sores Hematopoietic Stem Cells Wound Healing

Keywords

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Chronic wounds Pressure sores Hematopoietic stem cells Wound healing

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

BASIC_SCIENCE

Blinding Strategy

SINGLE

Participants

Interventions

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Stem cell therapy

Injection of a hematopoietic stem cell suspension (50'000 CD 34+ cells per microliter) into the wound (1 microliter per cm2 of wound surface)

Intervention Type BIOLOGICAL

Eligibility Criteria

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Inclusion Criteria

* Para- or tetraplegic
* Sacral pressure sores
* Grade III-IV according to classification of Daniel and Seiler

Exclusion Criteria

* Diabetes mellitus
* Peripheral vascular disease
* Coronary artery disease
* Smoking
* Steroids and other immunosuppressive drugs
* Systemic autoimmune or rheumatoid diseases
* HIV
* Hepatitis B/C
Minimum Eligible Age

18 Years

Maximum Eligible Age

50 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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University Hospital, Basel, Switzerland

OTHER

Sponsor Role lead

Principal Investigators

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Oliver Scheufler, MD, PhD

Role: STUDY_DIRECTOR

University Hospital Basel, Spitalstrasse 21, 4031 Basel, Switzerland

Locations

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Swiss Paraplegic Center

Nottwil, , Switzerland

Site Status RECRUITING

Countries

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Switzerland

Central Contacts

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Oliver Scheufler, MD, PhD

Role: CONTACT

Phone: +41-61-265 2525

Email: [email protected]

References

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Wettstein R, Savic M, Pierer G, Scheufler O, Haug M, Halter J, Gratwohl A, Baumberger M, Schaefer DJ, Kalbermatten DF. Progenitor cell therapy for sacral pressure sore: a pilot study with a novel human chronic wound model. Stem Cell Res Ther. 2014 Jan 29;5(1):18. doi: 10.1186/scrt407.

Reference Type DERIVED
PMID: 24476740 (View on PubMed)

Other Identifiers

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552

Identifier Type: -

Identifier Source: org_study_id