Trial Outcomes & Findings for Primary Vaccination Study With a Pneumococcal Conjugate Vaccine in Healthy Children 6 to 8 Weeks of Age (NCT NCT00533507)

NCT ID: NCT00533507

Last Updated: 2018-06-08

Results Overview

Concentrations are given as geometric mean concentrations (GMC) and expressed in Enzyme-Linked Immuno Sorbent Assay (ELISA) units per milliliter (EL.U/mL).

Recruitment status

COMPLETED

Study phase

PHASE3

Target enrollment

230 participants

Primary outcome timeframe

One month after the third dose

Results posted on

2018-06-08

Participant Flow

Participant milestones

Participant milestones
Measure
Synflorix Group
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Overall Study
STARTED
230
Overall Study
COMPLETED
229
Overall Study
NOT COMPLETED
1

Reasons for withdrawal

Reasons for withdrawal
Measure
Synflorix Group
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Overall Study
Withdrawal by Subject
1

Baseline Characteristics

Primary Vaccination Study With a Pneumococcal Conjugate Vaccine in Healthy Children 6 to 8 Weeks of Age

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Synflorix Group
n=230 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Age, Continuous
6.4 weeks
STANDARD_DEVIATION 0.60 • n=5 Participants
Sex: Female, Male
Female
115 Participants
n=5 Participants
Sex: Female, Male
Male
115 Participants
n=5 Participants

PRIMARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Concentrations are given as geometric mean concentrations (GMC) and expressed in Enzyme-Linked Immuno Sorbent Assay (ELISA) units per milliliter (EL.U/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=219 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Concentration of Anti-Protein D Antibodies
2277.6 EL.U/mL
Interval 2048.7 to 2532.1

PRIMARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Concentrations are given as geometric mean titers (GMC) and expressed in microgram per milliliter (µg/mL). The vaccine pneumococcal serotypes assessed include 1, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, and 23F.

Outcome measures

Outcome measures
Measure
Synflorix Group
n=219 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Concentration of Anti-Pneumococcal Antibodies
Anti-1
2.92 µg/mL
Interval 2.65 to 3.22
Concentration of Anti-Pneumococcal Antibodies
Anti-4
3.79 µg/mL
Interval 3.42 to 4.19
Concentration of Anti-Pneumococcal Antibodies
Anti-5
4.50 µg/mL
Interval 4.11 to 4.93
Concentration of Anti-Pneumococcal Antibodies
Anti-6B
1.69 µg/mL
Interval 1.47 to 1.94
Concentration of Anti-Pneumococcal Antibodies
Anti-7F
4.07 µg/mL
Interval 3.72 to 4.46
Concentration of Anti-Pneumococcal Antibodies
Anti-9V
3.90 µg/mL
Interval 3.51 to 4.32
Concentration of Anti-Pneumococcal Antibodies
Anti-14
5.69 µg/mL
Interval 5.1 to 6.35
Concentration of Anti-Pneumococcal Antibodies
Anti-18C
7.28 µg/mL
Interval 6.43 to 8.25
Concentration of Anti-Pneumococcal Antibodies
Anti-19F
8.04 µg/mL
Interval 7.37 to 8.78
Concentration of Anti-Pneumococcal Antibodies
Anti-23F
2.81 µg/mL
Interval 2.44 to 3.22

SECONDARY outcome

Timeframe: Before the first dose (pre) and one month after (post) the third dose

Population: Analysis was performed on the According-to-Protocol (ATP) cohort for analysis of immunogenicity, on subjects with available results

Anti-protein D antibody cut-off value assessed was greater than or equal to 100 Enzyme-Linked Immuno Sorbent Assay (ELISA) units per milliliter (EL.U/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=219 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Anti-Protein D Antibody Concentrations Above the Cut-Off Value
Pre (N=217)
38 subjects
Number of Subjects With Anti-Protein D Antibody Concentrations Above the Cut-Off Value
Post (N=219)
218 subjects

SECONDARY outcome

Timeframe: Before the first dose (pre) and one month after (post) the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-pneumococcal antibody cut-off value assessed was 0.05 microgram per milliliter (μg/mL). The vaccine pneumococcal serotypes assessed include 1, 4, 5, 6B, 7F, 9V, 14, 18C, 19F, and 23F.

Outcome measures

Outcome measures
Measure
Synflorix Group
n=219 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-19F Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-23F Pre (N=219)
91 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-23F Post (N=219)
216 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-1 Pre (N=217)
111 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-1 Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-4 Pre (N=217)
85 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-4 Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-5 Pre (N=217)
149 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-5 Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-6B Pre (N=217)
107 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-6B Post (N=219)
215 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-7F Pre (N=218)
138 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-7F Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-9V Pre (N=218)
123 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-9V Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-14 Pre (N=218)
203 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-14 Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-18C Pre (N=219)
153 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-18C Post (N=219)
219 subjects
Number of Subjects With Vaccine Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-19F Pre (N=219)
172 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-pneumococcal antibody cut-off value assessed was 0.05 microgram per milliliter (µg/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=219 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Cross-Reactive Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-6A (N=219)
210 subjects
Number of Subjects With Cross-Reactive Pneumococcal Serotype Antibody Concentrations Above the Cut-Off Value
Anti-19A (N=218)
200 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Cut-off value for opsonophagocytic activity against pneumococcal antibody assessed was greater than or equal to 1:8 titer.

Outcome measures

Outcome measures
Measure
Synflorix Group
n=103 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-1 (N=102)
98 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-4 (N=103)
103 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-5 (N=102)
101 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-6B (N=102)
89 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-7F (N=103)
103 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-9V (N=98)
98 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-14 (N=102)
101 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-18C (N=101)
99 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-19F (N=103)
101 subjects
Number of Subjects With Opsonophagocytic Activity Against Vaccine Pneumococcal Serotypes Above the Cut-Off Value
Opsono-23F (N=102)
98 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Cut-off value for opsonophagocytic activity against pneumococcal antibody assessed was greater than or equal to 1:8 titer.

Outcome measures

Outcome measures
Measure
Synflorix Group
n=97 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Opsonophagocytic Activity Against Cross-Reactive Pneumococcal Serotypes Above the Cut-Off Value
Opsono-6A
87 subjects
Number of Subjects With Opsonophagocytic Activity Against Cross-Reactive Pneumococcal Serotypes Above the Cut-Off Value
Opsono-19A
38 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-polyribosyl-ribitol phosphate antibody cut-off value assessed was greater than or equal to 0.15 microgram per milliliter (μg/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=58 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Anti-Polyribosyl-Ribitol Phosphate Antibody Concentrations Above the Cut-Off Value
58 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-diphteria and anti-tetanus toxoids antibody cut-off values assessed were greater than or equal to 0.10 International Units per milliliter (IU/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=58 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Anti-Diphteria and Anti-Tetanus Toxoids Antibody Concentrations Above the Cut-Off Value
Anti-diphteria toxoid
58 subjects
Number of Subjects With Anti-Diphteria and Anti-Tetanus Toxoids Antibody Concentrations Above the Cut-Off Value
Anti-tetanus toxoid
58 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-PT, anti-FHA and anti-PRN cut-off values assessed were greater than or equal to 5 Enzyme-Linked Immuno Sorbent Assay (ELISA) units per milliliter (EL.U/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=58 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Anti-Pertussis (PT), Anti-Filamentous Hemagglutinin (FHA) and Anti-Pertactin (PRN) Antibody Concentrations Above the Cut-Off Value
Anti-PT (N=58)
58 subjects
Number of Subjects With Anti-Pertussis (PT), Anti-Filamentous Hemagglutinin (FHA) and Anti-Pertactin (PRN) Antibody Concentrations Above the Cut-Off Value
Anti-FHA (N=58)
58 subjects
Number of Subjects With Anti-Pertussis (PT), Anti-Filamentous Hemagglutinin (FHA) and Anti-Pertactin (PRN) Antibody Concentrations Above the Cut-Off Value
Anti-PRN (N=57)
57 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-HBs antibody cut-off value assessed was greater than or equal to 10 milli-International Units per milliliter (mIU/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=32 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Anti-Hepatitis B Surface Antigen (HBs) Antibody Concentrations Above the Cut-Off Value
32 subjects

SECONDARY outcome

Timeframe: One month after the third dose

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-poliovirus 1, 2 and 3 antibody cut-off value assessed was greater than or equal to 1:8 titer.

Outcome measures

Outcome measures
Measure
Synflorix Group
n=44 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Anti-Poliovirus 1, 2 and 3 Antibody Titers Above the Cut-Off Value
Anti-poliovirus 1
44 subjects
Number of Subjects With Anti-Poliovirus 1, 2 and 3 Antibody Titers Above the Cut-Off Value
Anti-poliovirus 2
44 subjects
Number of Subjects With Anti-Poliovirus 1, 2 and 3 Antibody Titers Above the Cut-Off Value
Anti-poliovirus 3
44 subjects

SECONDARY outcome

Timeframe: Four months after the administration of the second dose of Rotarix™ vaccine

Population: Analysis was performed on ATP cohort for analysis of immunogenicity, on subjects with available results

Anti-rotavirus IgA antibody cut-off value assessed was greater than or equal to 20 Units per milliliter (U/mL).

Outcome measures

Outcome measures
Measure
Synflorix Group
n=51 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects With Anti-rotavirus Immunoglobulin A Antibody Concentrations Above the Cut-Off Value
44 subjects

SECONDARY outcome

Timeframe: During the 4-day (Day 0-3) period after each dose

Solicited local symptoms assessed include pain, redness and swelling. Solicited general symptoms assessed include diarrhoea, drowsiness, fever, irritability, loss of appetite, and vomiting

Outcome measures

Outcome measures
Measure
Synflorix Group
n=230 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects Reporting Solicited Symptoms
Pain
146 subjects
Number of Subjects Reporting Solicited Symptoms
Redness
144 subjects
Number of Subjects Reporting Solicited Symptoms
Swelling
139 subjects
Number of Subjects Reporting Solicited Symptoms
Diarrhoea
7 subjects
Number of Subjects Reporting Solicited Symptoms
Drowsiness
190 subjects
Number of Subjects Reporting Solicited Symptoms
Fever
153 subjects
Number of Subjects Reporting Solicited Symptoms
Irritability
203 subjects
Number of Subjects Reporting Solicited Symptoms
Loss of appetite
159 subjects
Number of Subjects Reporting Solicited Symptoms
Vomiting
55 subjects

SECONDARY outcome

Timeframe: During the 31-day (Day 0-30) period after each dose

An AE is any untoward medical occurrence in a clinical investigation subject, temporally associated with the use of a medicinal product, whether or not considered related to the medicinal product

Outcome measures

Outcome measures
Measure
Synflorix Group
n=230 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects Reporting Unsolicited Adverse Events (AE)
95 subjects

SECONDARY outcome

Timeframe: Up to one month after the third dose

An SAE is any untoward medical occurrence that: results in death, is life-threatening, requires hospitalization or prolongation of existing hospitalization, results in disability/incapacity, is a congenital anomaly/birth defect in the offspring of a study subject, or may evolve into one of the outcomes listed above.

Outcome measures

Outcome measures
Measure
Synflorix Group
n=230 Participants
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Number of Subjects Reporting Serious Adverse Events (SAE)
15 subjects

Adverse Events

Synflorix Group

Serious events: 15 serious events
Other events: 224 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Synflorix Group
n=230 participants at risk
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Infections and infestations
Urinary tract infection
2.6%
6/230
Infections and infestations
Bronchiolitis
1.7%
4/230
Infections and infestations
Gastroenteritis
0.87%
2/230
General disorders
Pyrexia
0.87%
2/230
Respiratory, thoracic and mediastinal disorders
Bronchial hyperreactivity
0.43%
1/230
Infections and infestations
Bronchopneumonia
0.43%
1/230
Metabolism and nutrition disorders
Dehydration
0.43%
1/230
Skin and subcutaneous tissue disorders
Dermatitis atopic
0.43%
1/230
Skin and subcutaneous tissue disorders
Dermatitis diaper
0.43%
1/230
Gastrointestinal disorders
Enterocolitis
0.43%
1/230
Infections and infestations
Gastroenteritis bacterial
0.43%
1/230
Infections and infestations
Kawasaki's disease
0.43%
1/230
Infections and infestations
Meningitis aseptic
0.43%
1/230
Infections and infestations
Upper respiratory tract infection
0.43%
1/230
Renal and urinary disorders
Vesicoureteric reflux
0.43%
1/230

Other adverse events

Other adverse events
Measure
Synflorix Group
n=230 participants at risk
Subjects receiving Synflorix co-administered with Infanrix™ hexa at 1.5, 3 and 6 months of age, and co-administered with Rotarix™ at 1.5 and 3 months of age.
Infections and infestations
Upper respiratory tract infection
23.5%
54/230
General disorders
Pain
63.5%
146/230
General disorders
Redness
62.6%
144/230
General disorders
Swelling
60.4%
139/230
General disorders
Drowsiness
82.6%
190/230
General disorders
Fever
66.5%
153/230
General disorders
Irritability
88.3%
203/230
General disorders
Loss of appetite
69.1%
159/230
General disorders
Vomiting
23.9%
55/230

Additional Information

GSK Response Center

GlaxoSmithKline

Phone: 866-435-7343

Results disclosure agreements

  • Principal investigator is a sponsor employee GSK agreements may vary with individual investigators, but will not prohibit any investigator from publishing. GSK supports the publication of results from all centers of a multi-center trial but requests that reports based on single-site data not precede the primary publication of the entire clinical trial.
  • Publication restrictions are in place

Restriction type: OTHER