Interleukin-7 in Treating Patients With Metastatic Melanoma or Locally Advanced or Metastatic Kidney Cancer

NCT ID: NCT00492440

Last Updated: 2012-10-18

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

PHASE1

Total Enrollment

9 participants

Study Classification

INTERVENTIONAL

Study Start Date

2007-05-31

Study Completion Date

2010-12-31

Brief Summary

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RATIONALE: Interleukin-7 may stimulate the white blood cells to kill tumor cells.

PURPOSE: This phase I trial is studying the side effects and best dose of interleukin-7 in treating patients with metastatic melanoma or locally advanced or metastatic kidney cancer.

Detailed Description

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OBJECTIVES:

Primary

* Determine the safety of recombinant interleukin-7 (IL-7) in patients with metastatic melanoma or locally advanced or metastatic renal cell carcinoma.
* Confirm the previously documented safety profile of non-glycosylated IL-7 in these patients.
* Determine the safety of higher doses of recombinant IL-7 in these patients.
* Determine the maximum tolerated dose of recombinant IL-7 in these patients.
* Determine the biologically active dose of recombinant IL-7 in these patients.

Secondary

* Determine the pharmacokinetics and pharmacodynamics of recombinant IL-7 in these patients.
* Compare the biological and clinical effects of recombinant IL-7 with non-glycosylated IL-7 in these patients.
* Determine the potential antitumor effect of recombinant IL-7 in these patients.
* Determine the dose and administration schedule of recombinant IL-7 in these patients.

OUTLINE: This is a dose-escalation study. Patients are stratified according to lymphocyte count (normal lymphocyte count \[CD4+ T cells \> 400/mm\^3\] vs lymphopenic \[CD4+ T cells \< 400/mm\^3\]). Patients are assigned to 1 of 2 treatment groups.

* Group 1 (normal lymphocyte count): Patients receive recombinant interleukin-7 (IL-7) subcutaneously once a week (to determine an active dose) for up to 3 weeks in the absence of disease progression or unacceptable toxicity.
* Group 2 (lymphopenic): Patients receive recombinant IL-7 subcutaneously once a week for up to 3 weeks at one dose level below the active dose determined in group 1.

Cohorts of 3-6 patients from each group receive escalating doses of recombinant IL-7 until the maximum tolerated dose (MTD) is determined. The MTD is the defined as the dose preceding that at which 2 of 3 or 2 of 6 patients experience dose-limiting toxicity. Up to 30 additional patients may be treated at the MTD.

Patients undergo blood and bone marrow collection periodically for pharmacokinetic, pharmacodynamic, and immunological studies. Samples are analyzed for the presence of antibodies and proteins via ELISA; CD3, CD4, and CD8 T cell counts, CD127, Ki-67, and Bcl-2 expression in CD4+ and CD8+ T cells, and CD19 B cell counts via flow cytometry; and clonal B cell proliferation via PCR and flow cytometry.

After completion of study treatment, patients are followed at 3 months.

Conditions

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Kidney Cancer Melanoma (Skin)

Keywords

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recurrent renal cell cancer stage IV renal cell cancer stage III renal cell cancer recurrent melanoma stage IV melanoma

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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CYT107 (r-hIL-7)

Group Type EXPERIMENTAL

recombinant interleukin-7

Intervention Type BIOLOGICAL

gene expression analysis

Intervention Type GENETIC

polymerase chain reaction

Intervention Type GENETIC

protein expression analysis

Intervention Type GENETIC

flow cytometry

Intervention Type OTHER

immunoenzyme technique

Intervention Type OTHER

immunologic technique

Intervention Type OTHER

laboratory biomarker analysis

Intervention Type OTHER

pharmacological study

Intervention Type OTHER

Interventions

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recombinant interleukin-7

Intervention Type BIOLOGICAL

gene expression analysis

Intervention Type GENETIC

polymerase chain reaction

Intervention Type GENETIC

protein expression analysis

Intervention Type GENETIC

flow cytometry

Intervention Type OTHER

immunoenzyme technique

Intervention Type OTHER

immunologic technique

Intervention Type OTHER

laboratory biomarker analysis

Intervention Type OTHER

pharmacological study

Intervention Type OTHER

Eligibility Criteria

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Inclusion Criteria

* Measurable or evaluable disease
* Previously received high-dose interleukin-2 OR have a contraindication for this treatment
* No previously untreated or unstable brain metastases
* No splenic metastasis

PATIENT CHARACTERISTICS:

* ECOG performance status 0-2
* Life expectancy ≥ 3 months
* Absolute neutrophil count \> 1,000/mm\^3
* Platelet count \> 100,000/mm\^3
* PT/PTT ≤ 1.5 times upper limit of normal (ULN)
* Creatinine \< 1.5 times ULN
* AST and ALT \< 2.5 times ULN
* Conjugated (Direct) bilirubin ≤ 1.25 times ULN
* Not pregnant or nursing
* Negative pregnancy test
* Fertile patients must use effective contraception
* LVEF ≥ 45% by cardiac stress test (e.g., stress thallium, stress MUGA, dobutamine echocardiogram, or other stress test) for patients meeting any of the following criteria:

* History of ECG abnormalities
* Symptoms of cardiac ischemia
* At least 50 years of age and over
* Familial or personal history of heart failure
* Previously treated with antimitotic agents susceptible to trigger heart failure
* FEV\_1 \> 60% of predicted (for patients with a prolonged smoking history or symptoms of respiratory dysfunction)
* No concurrent cognitive impairment or likelihood of developing cognitive impairment on study therapy
* No concurrent splenomegaly or proliferative hematologic disease
* No documented HIV positivity
* No acute hepatitis A or hepatitis B or C

* Positive hepatitis B serology indicative of previous immunization (i.e., HBs Ab positive and HBc Ab negative) allowed
* Positive hepatitis C serology allowed provided HCV RNA load by PCR is negative
* Resting blood pressure ≤ 140/90 mm Hg on standard antihypertensive therapy

* Untreated hypertensive patients who received standard antihypertensive therapy allowed provided hypertension is well controlled
* No QTc prolongation ≥ 470 msec
* No prior history of cardiovascular disease, arrhythmias, or significant ECG abnormalities
* No active infection requiring systemic treatment and/or hospitalization within the past 28 days

* Patients who have completed therapy or are clinically stable on therapy, in the opinion of the investigator, are eligible
* No history of autoimmune disease
* No history of severe asthma
* No history of medical or psychiatric disease that would preclude study treatment
* No documented cirrhosis or documented acute hepatitis

PRIOR CONCURRENT THERAPY:

* See Disease Characteristics
* More than 2 weeks since prior systemic corticosteroid therapy
* More than 4 weeks since prior and no other concurrent cytotoxic therapy, immunotherapy, biological agents (i.e., cytokines, growth factors, or monoclonal antibodies), or antitumor vaccines
* More than 7 days since prior hepatotoxic drugs unless medically necessary
* More than 2 days since prior alcohol consumption
* More than 1 day since prior acetaminophen use
* No prior splenectomy
* No prior allogeneic hematopoietic stem cell transplantation or solid organ transplantation
* No concurrent palliative therapy
* No concurrent chemotherapy
* No concurrent chronic anticoagulation (i.e., high-dose warfarin or heparin)

* Warfarin dose 1 to 2 mg/day allowed
* No concurrent chronic medications for asthma
* No other concurrent investigational agents
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Cytheris, Inc.

INDUSTRY

Sponsor Role collaborator

Cytheris SA

INDUSTRY

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Steven A. Rosenberg, MD, PhD

Role: STUDY_CHAIR

NCI - Surgery Branch

Locations

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Warren Grant Magnuson Clinical Center - NCI Clinical Trials Referral Office

Bethesda, Maryland, United States

Site Status

Countries

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United States

Other Identifiers

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07-C-0114

Identifier Type: OTHER

Identifier Source: secondary_id

CLI-107-04

Identifier Type: OTHER

Identifier Source: secondary_id

CLI-107-04

Identifier Type: -

Identifier Source: org_study_id