PROS-1-Male Hormonal Contraceptive Regimens on Prostate Tissue

NCT ID: NCT00490555

Last Updated: 2013-11-15

Study Results

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Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2/PHASE3

Total Enrollment

32 participants

Study Classification

INTERVENTIONAL

Study Start Date

2009-01-31

Study Completion Date

2012-03-31

Brief Summary

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The investigators propose to examine the in vivo responses to hormonal manipulation at the molecular level directly in the tissue of interest (prostate). As in the investigators' previous, pilot study, the investigators will use the novel approach of procuring tissue specimens from normal, healthy men who might be chose to use a male hormonal contraceptive regimen were it available. The investigators will employ state of the art techniques such as laser capture microdissection (LCM) and cDNA microarrays to determine the tissue-specific consequences of male hormonal contraceptive regimens on the prostate. The results will help guide the design, safety monitoring, and selection of male hormonal contraceptive agents and provide valuable insights into prostate human prostate biology.

The investigators will test the hypothesis that exogenous T administration that results in increased circulating T and dihydrotestosterone (DHT) levels will increase intraprostatic concentrations of T and its metabolite DHT.

The investigators will test the hypothesis that the addition of a potent 5α-reductase inhibitor, dutasteride, or the progestin, Depomedoxyprogesterone (IM DMPA), to T administration in young and middle aged men will decrease intraprostatic DHT and increase intraprostatic T concentrations compared to T alone.

The investigators will test the hypothesis that the addition of a 5α-reductase inhibitor dutasteride or the progestin IM DMPA to exogenous T, by reducing intraprostatic DHT, will decrease prostate epithelial proliferation, assessed by Ki-67 labeling index (Ki-67LI), and increase apoptosis, assessed by caspase-3 expression, and decrease androgen-regulated protein expression such as prostate specific antigen (PSA).

The investigators will test the hypothesis that the addition of a 5α-reductase inhibitor or the progestin IM DMPA to exogenous T, by modifying the intraprostatic hormonal milieu, will alter prostate epithelial gene expression. Specifically, the investigators expect that the addition of the 5α-reductase inhibitor dutasteride or the progestin IM DMPA to exogenous T, will result in decreased expression of androgen-regulated genes such as PSA.

Detailed Description

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The purpose of this research study is to understand the effects of testosterone on the prostate. This knowledge will be used to help in the development of a safe male hormonal contraception.

We will be administering three drugs in this study: Testim (testosterone (T) gel), dutasteride (which affects testosterone break down) and Depomedoxyprogesterone (DMPA, a progestin). We want to see their effects on levels of hormones in the blood and prostate. In addition, we will be examining the effects of these drugs on the expression of genes within the prostate. DMPA suppresses LH and FSH, which are hormones made by the pituitary gland, thus blocking the signal from the brain that causes the testes to make testosterone. Prolonged (\> 1 month) low levels of LH and FSH cause decreased sperm production in normal men. However, men may experience some side effects from the low levels of testosterone caused by DMPA; adding testosterone to DMPA eliminates these side effects while more effectively blocking LH and FSH release and sperm production. This combination of drugs is a promising male contraceptive regimen. However, the effect of these drugs on the prostate is not known. Some studies suggest that testosterone administration may promote prostate growth. Dutasteride blocks the conversion of testosterone to dihydrotestosterone and is used to treat men with enlarged prostates. Dutasteride shrinks the prostate. It is possible that combining testosterone and dutasteride may be an effective part of a male hormonal contraceptive regime. Therefore, further studies examining the effect of testosterone, DMPA and dutasteride on the prostate are needed.

Conditions

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Healthy

Keywords

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Male Contraception Testosterone Testis Contraception Prostate

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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1

Placebo gel + Placebo pill + placebo injection

Group Type PLACEBO_COMPARATOR

Placebo Testosterone gel

Intervention Type OTHER

Place gel applied daily for 12 weeks

Placebo dutasteride

Intervention Type OTHER

placebo pill for 12 weeks

Placebo DMPA

Intervention Type OTHER

placebo DMPA injection Once

2

Testosterone 1% transdermal gel 10 g + placebo pill + placebo injection

Group Type ACTIVE_COMPARATOR

Testosterone gel

Intervention Type DRUG

Testosterone gel 10 g

3

Testosterone 1% transdermal gel 10 g + dutasteride 0.5 mg Orally + placebo injection

Group Type ACTIVE_COMPARATOR

Testosterone gel

Intervention Type DRUG

Testosterone gel 10 g

Dutasteride

Intervention Type DRUG

dutasteride 0.5 mg orally

4

Testosterone 1% transdermal gel 10 g + placebo pill + DMPA 300 mg injection (IM)

Group Type ACTIVE_COMPARATOR

Testosterone gel

Intervention Type DRUG

Testosterone gel 10 g

Depo-Medroxyprogesterone (DMPA)

Intervention Type DRUG

300 mg DMPA injection on Day 0 IM (into the muscle)

Placebo dutasteride

Intervention Type OTHER

placebo pill for 12 weeks

Interventions

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Testosterone gel

Testosterone gel 10 g

Intervention Type DRUG

Dutasteride

dutasteride 0.5 mg orally

Intervention Type DRUG

Depo-Medroxyprogesterone (DMPA)

300 mg DMPA injection on Day 0 IM (into the muscle)

Intervention Type DRUG

Placebo Testosterone gel

Place gel applied daily for 12 weeks

Intervention Type OTHER

Placebo dutasteride

placebo pill for 12 weeks

Intervention Type OTHER

Placebo DMPA

placebo DMPA injection Once

Intervention Type OTHER

Other Intervention Names

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Testim Avodart Depo-Provera

Eligibility Criteria

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Inclusion Criteria

* Men in good health, and without a history of chronic androgen therapy or known history of gonadal or prostate abnormalities.

* PSA ≤ 2.
* Age 25-55 years
* Ability to understand the study,study procedures and provide consent
* Normal serum total T, LH, FSH, urine analyses, and sperm count \> or equal to 15million/ml
* International Prostate Symptom Score (IPSS) \< 10
* Normal seminal fluid analysis (\>20 million sperm/ml)
* Agree not to donate blood during the treatment and recovery periods

Exclusion Criteria

* A history or evidence of prostate or breast cancer
* History of invasive therapy for BPH
* History of acute urinary retention
* Current or past treatment with a 5α-reductase inhibitor
* History of anti/androgenic drugs or drugs that interfere with steroid metabolism within past 3 months
* Severe systemic illness (renal, liver, cardiac, lung disease, cancer, poorly controlled diabetes)
* Known untreated obstructive sleep apnea
* Hematocrit \> 52%
* Skin disease that might interfere with T gel absorption
* Hypersensitivity to any of the drugs used in the study
* History of a bleeding disorder or anticoagulation
* History of drug or alcohol abuse within 12 months
* History of infertility or desire for fertility within 12 months, or current pregnant partner
* A first-degree relative (i.e. father, brother) with a history of prostate cancer
* Abnormal digital rectal examination or prostate ultrasound
Minimum Eligible Age

25 Years

Maximum Eligible Age

55 Years

Eligible Sex

MALE

Accepts Healthy Volunteers

Yes

Sponsors

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Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)

NIH

Sponsor Role collaborator

University of Washington

OTHER

Sponsor Role lead

Responsible Party

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Stephanie T. Page

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Stephanie T Page, MD, PhD

Role: PRINCIPAL_INVESTIGATOR

University of Washington

Locations

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University of Washington

Seattle, Washington, United States

Site Status

Countries

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United States

References

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Amory JK, Page ST, Bremner WJ. Drug insight: Recent advances in male hormonal contraception. Nat Clin Pract Endocrinol Metab. 2006 Jan;2(1):32-41. doi: 10.1038/ncpendmet0069.

Reference Type BACKGROUND
PMID: 16932251 (View on PubMed)

Brady BM, Amory JK, Perheentupa A, Zitzmann M, Hay CJ, Apter D, Anderson RA, Bremner WJ, Pollanen P, Nieschlag E, Wu FC, Kersemaekers WM. A multicentre study investigating subcutaneous etonogestrel implants with injectable testosterone decanoate as a potential long-acting male contraceptive. Hum Reprod. 2006 Jan;21(1):285-94. doi: 10.1093/humrep/dei300. Epub 2005 Sep 19.

Reference Type BACKGROUND
PMID: 16172147 (View on PubMed)

Burkman R, Schlesselman JJ, Zieman M. Safety concerns and health benefits associated with oral contraception. Am J Obstet Gynecol. 2004 Apr;190(4 Suppl):S5-22. doi: 10.1016/j.ajog.2004.01.061.

Reference Type BACKGROUND
PMID: 15105794 (View on PubMed)

Jacobsen SJ, Girman CJ, Lieber MM. Natural history of benign prostatic hyperplasia. Urology. 2001 Dec;58(6 Suppl 1):5-16; discussion 16. doi: 10.1016/s0090-4295(01)01298-5.

Reference Type BACKGROUND
PMID: 11750242 (View on PubMed)

Jemal A, Siegel R, Ward E, Murray T, Xu J, Thun MJ. Cancer statistics, 2007. CA Cancer J Clin. 2007 Jan-Feb;57(1):43-66. doi: 10.3322/canjclin.57.1.43.

Reference Type BACKGROUND
PMID: 17237035 (View on PubMed)

Wilson JD, George FW. The Physiology of Reproduction. Raven Press, 1994

Reference Type BACKGROUND

Russell DW, Wilson JD. Steroid 5 alpha-reductase: two genes/two enzymes. Annu Rev Biochem. 1994;63:25-61. doi: 10.1146/annurev.bi.63.070194.000325. No abstract available.

Reference Type BACKGROUND
PMID: 7979239 (View on PubMed)

Thompson IM, Goodman PJ, Tangen CM, Lucia MS, Miller GJ, Ford LG, Lieber MM, Cespedes RD, Atkins JN, Lippman SM, Carlin SM, Ryan A, Szczepanek CM, Crowley JJ, Coltman CA Jr. The influence of finasteride on the development of prostate cancer. N Engl J Med. 2003 Jul 17;349(3):215-24. doi: 10.1056/NEJMoa030660. Epub 2003 Jun 24.

Reference Type BACKGROUND
PMID: 12824459 (View on PubMed)

Geller J. Effect of finasteride, a 5 alpha-reductase inhibitor on prostate tissue androgens and prostate-specific antigen. J Clin Endocrinol Metab. 1990 Dec;71(6):1552-5. doi: 10.1210/jcem-71-6-1552.

Reference Type BACKGROUND
PMID: 1699965 (View on PubMed)

Geller J, Albert J. Effects of castration compared with total androgen blockade on tissue dihydrotestosterone (DHT) concentration in benign prostatic hyperplasia (BPH). Urol Res. 1987;15(3):151-3. doi: 10.1007/BF00254427.

Reference Type BACKGROUND
PMID: 3629749 (View on PubMed)

Mohler JL, Gregory CW, Ford OH 3rd, Kim D, Weaver CM, Petrusz P, Wilson EM, French FS. The androgen axis in recurrent prostate cancer. Clin Cancer Res. 2004 Jan 15;10(2):440-8. doi: 10.1158/1078-0432.ccr-1146-03.

Reference Type BACKGROUND
PMID: 14760063 (View on PubMed)

Forti G, Salerno R, Moneti G, Zoppi S, Fiorelli G, Marinoni T, Natali A, Costantini A, Serio M, Martini L, et al. Three-month treatment with a long-acting gonadotropin-releasing hormone agonist of patients with benign prostatic hyperplasia: effects on tissue androgen concentration, 5 alpha-reductase activity and androgen receptor content. J Clin Endocrinol Metab. 1989 Feb;68(2):461-8. doi: 10.1210/jcem-68-2-461.

Reference Type BACKGROUND
PMID: 2465302 (View on PubMed)

Habib FK, Ross M, Tate R, Chisholm GD. Differential effect of finasteride on the tissue androgen concentrations in benign prostatic hyperplasia. Clin Endocrinol (Oxf). 1997 Feb;46(2):137-44. doi: 10.1046/j.1365-2265.1997.950908.x.

Reference Type BACKGROUND
PMID: 9135694 (View on PubMed)

Page ST, Lin DW, Mostaghel EA, Hess DL, True LD, Amory JK, Nelson PS, Matsumoto AM, Bremner WJ. Persistent intraprostatic androgen concentrations after medical castration in healthy men. J Clin Endocrinol Metab. 2006 Oct;91(10):3850-6. doi: 10.1210/jc.2006-0968. Epub 2006 Aug 1.

Reference Type BACKGROUND
PMID: 16882745 (View on PubMed)

Page ST, Amory JK, Anawalt BD, Irwig MS, Brockenbrough AT, Matsumoto AM, Bremner WJ. Testosterone gel combined with depomedroxyprogesterone acetate is an effective male hormonal contraceptive regimen and is not enhanced by the addition of a GnRH antagonist. J Clin Endocrinol Metab. 2006 Nov;91(11):4374-80. doi: 10.1210/jc.2006-1411. Epub 2006 Aug 29.

Reference Type BACKGROUND
PMID: 16940442 (View on PubMed)

Other Identifiers

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RFA-HD-06-014;

Identifier Type: -

Identifier Source: secondary_id

06-4795-A 01

Identifier Type: OTHER

Identifier Source: secondary_id

31434-A

Identifier Type: -

Identifier Source: org_study_id