Non-Myeloablative Bone Marrow Transplant for Patients With Sickle Cell Anemia and Other Blood Disorders

NCT ID: NCT00489281

Last Updated: 2019-04-17

Study Results

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Basic Information

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Recruitment Status

TERMINATED

Clinical Phase

PHASE2

Total Enrollment

43 participants

Study Classification

INTERVENTIONAL

Study Start Date

2008-06-23

Study Completion Date

2018-12-29

Brief Summary

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RATIONALE: Giving low doses of chemotherapy, such as fludarabine and cyclophosphamide, and total-body irradiation before a donor bone marrow transplant helps stop the growth of abnormal cells. It also helps stop the patient's immune system from rejecting the donor's stem cells. When the healthy stem cells from a donor are infused into the patient they may help the patient's bone marrow make stem cells, red blood cells, white blood cells, and platelets. Sometimes the transplanted cells from a donor can make an immune response against the body's normal cells. Giving sirolimus and mycophenolate mofetil after transplant may stop this from happening.

PURPOSE: This phase II trial is studying how well giving fludarabine and cyclophosphamide together with total-body irradiation followed by a donor bone marrow transplant works in treating patients with sickle cell anemia and other blood disorders.

Detailed Description

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OBJECTIVES:

* Determine the transplant-related mortality and progression-free survival of patients with severe hemoglobinopathies receiving nonmyeloablative conditioning comprising fludarabine phosphate, cyclophosphamide, and total-body irradiation followed by partially HLA-mismatched bone marrow transplantation from first-degree relatives or HLA-matched donors.
* Characterize donor hematopoietic chimerism at 30, 60, and 180 days after transplantation in these patients.
* Determine the hematologic and non-hematologic toxicity of this regimen in these patients.

OUTLINE:

* Preparative regimen: Patients receive fludarabine phosphate IV over 30 minutes on days -6 to -2 and cyclophosphamide IV over 1-2 hours on days -6 and -5. Patients also undergo total-body irradiation on day -1.
* Bone marrow transplantation: Patients undergo allogeneic bone marrow transplantation on day 0. Patients then receive cyclophosphamide IV over 1-2 hours on days 3 and 4.
* Graft-versus-host disease prophylaxis: Patients receive sirolimus orally daily on days 5-365 and oral mycophenolate mofetil 3 times a day on days 5-35.

After completion of study treatment, patients are followed periodically.

PROJECTED ACCRUAL: A total of 50 patients will be accrued for this study.

Conditions

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Sickle Cell Disease

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Transplant - 200 cGy

Conditioning regimen with anti-thymocyte globulin, fludarabine, cyclophosphamide, and total body irradiation - 200. Seizure prophylaxis with levetiracetam. Allogeneic bone marrow transplant infusion on Day 0. Graft-vs-host-disease (GVHD) prophylaxis with cyclophosphamide, mycophenolate mofetil, and sirolimus.

Group Type EXPERIMENTAL

Cyclophosphamide

Intervention Type DRUG

Cyclophosphamide (Cy) 14.5 mg/kg/day intravenously (IV) on Days -6 and -5 and 50 mg/kg/day IV on Days +3 and +4.

Fludarabine

Intervention Type DRUG

Fludarabine 30 mg/m\^2/day IV on Days -6, -5, -4, -3, and -2.

Mycophenolate mofetil

Intervention Type DRUG

Mycophenolate mofetil 15 mg/kg by mouth (PO) three times a day from Day +5 to Day +35.

Sirolimus

Intervention Type DRUG

The first dose of Sirolimus is 6 mg PO on Day +5. Further dosing is adjusted according to drug levels. Sirolimus is continued through Day +365.

Allogeneic bone marrow transplant

Intervention Type PROCEDURE

An allogeneic bone marrow transplant is a procedure that involves taking bone marrow from a donor and giving it to a recipient.

Total body irradiation - 200

Intervention Type RADIATION

200 centigray (cGy) in one fraction on Day -1.

Levetiracetam

Intervention Type DRUG

Given at 500 mg PO twice daily from Day -6 to Day +365.

Anti-thymocyte globulin

Intervention Type BIOLOGICAL

Test dose of 0.5 mg/kg IV given on Day -9, then 2 mg/kg/day IV on Day -8 and -7.

Transplant - 400 cGy

Conditioning regimen with anti-thymocyte globulin, fludarabine, cyclophosphamide, and total body irradiation - 400. Seizure prophylaxis with levetiracetam. Allogeneic bone marrow transplant infusion on Day 0. Graft-vs-host-disease (GVHD) prophylaxis with cyclophosphamide, mycophenolate mofetil, and sirolimus.

Group Type EXPERIMENTAL

Cyclophosphamide

Intervention Type DRUG

Cyclophosphamide (Cy) 14.5 mg/kg/day intravenously (IV) on Days -6 and -5 and 50 mg/kg/day IV on Days +3 and +4.

Fludarabine

Intervention Type DRUG

Fludarabine 30 mg/m\^2/day IV on Days -6, -5, -4, -3, and -2.

Mycophenolate mofetil

Intervention Type DRUG

Mycophenolate mofetil 15 mg/kg by mouth (PO) three times a day from Day +5 to Day +35.

Sirolimus

Intervention Type DRUG

The first dose of Sirolimus is 6 mg PO on Day +5. Further dosing is adjusted according to drug levels. Sirolimus is continued through Day +365.

Allogeneic bone marrow transplant

Intervention Type PROCEDURE

An allogeneic bone marrow transplant is a procedure that involves taking bone marrow from a donor and giving it to a recipient.

Levetiracetam

Intervention Type DRUG

Given at 500 mg PO twice daily from Day -6 to Day +365.

Anti-thymocyte globulin

Intervention Type BIOLOGICAL

Test dose of 0.5 mg/kg IV given on Day -9, then 2 mg/kg/day IV on Day -8 and -7.

Total body irradiation - 400

Intervention Type RADIATION

400 centigray (cGy) in one fraction on Day -1.

Interventions

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Cyclophosphamide

Cyclophosphamide (Cy) 14.5 mg/kg/day intravenously (IV) on Days -6 and -5 and 50 mg/kg/day IV on Days +3 and +4.

Intervention Type DRUG

Fludarabine

Fludarabine 30 mg/m\^2/day IV on Days -6, -5, -4, -3, and -2.

Intervention Type DRUG

Mycophenolate mofetil

Mycophenolate mofetil 15 mg/kg by mouth (PO) three times a day from Day +5 to Day +35.

Intervention Type DRUG

Sirolimus

The first dose of Sirolimus is 6 mg PO on Day +5. Further dosing is adjusted according to drug levels. Sirolimus is continued through Day +365.

Intervention Type DRUG

Allogeneic bone marrow transplant

An allogeneic bone marrow transplant is a procedure that involves taking bone marrow from a donor and giving it to a recipient.

Intervention Type PROCEDURE

Total body irradiation - 200

200 centigray (cGy) in one fraction on Day -1.

Intervention Type RADIATION

Levetiracetam

Given at 500 mg PO twice daily from Day -6 to Day +365.

Intervention Type DRUG

Anti-thymocyte globulin

Test dose of 0.5 mg/kg IV given on Day -9, then 2 mg/kg/day IV on Day -8 and -7.

Intervention Type BIOLOGICAL

Total body irradiation - 400

400 centigray (cGy) in one fraction on Day -1.

Intervention Type RADIATION

Other Intervention Names

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Cytoxan Cy CTX Fludara MMF CellCept Rapamune Allo BMT TBI Keppra ATG Thymoglobulin TBI

Eligibility Criteria

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Inclusion Criteria

* Partially mismatched (at least haploidentical) first-degree relative donor available

* No minor (donor anti-recipient) ABO incompatibility if an ABO compatible donor is available

PATIENT CHARACTERISTICS:

* ECOG performance status (PS) 0-1 OR Karnofsky or Lansky PS 70-100%
* LVEF ≥ 35%
* FEV\_1 and forced vital capacity ≥ 40% predicted
* Direct bilirubin \< 3.1 mg/dL
* No moderate to severe pulmonary hypertension by ECHO
* No debilitating medical or psychiatric illness that would preclude study participation
* No HIV positivity
* Not pregnant or nursing
* Negative pregnancy test
* Fertile patients must use effective contraception

PRIOR CONCURRENT THERAPY:

* No prior transfusions from donor
* No immunosuppressive agents, including steroids as antiemetics, within 24 hours after the last dose of post-transplantation cyclophosphamide
Minimum Eligible Age

2 Years

Maximum Eligible Age

70 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Javier Bolanos-Meade, MD

Role: STUDY_CHAIR

Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins

Locations

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Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins

Baltimore, Maryland, United States

Site Status

Countries

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United States

References

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Bolanos-Meade J, Cooke KR, Gamper CJ, Ali SA, Ambinder RF, Borrello IM, Fuchs EJ, Gladstone DE, Gocke CB, Huff CA, Luznik L, Swinnen LJ, Symons HJ, Terezakis SA, Wagner-Johnston N, Jones RJ, Brodsky RA. Effect of increased dose of total body irradiation on graft failure associated with HLA-haploidentical transplantation in patients with severe haemoglobinopathies: a prospective clinical trial. Lancet Haematol. 2019 Apr;6(4):e183-e193. doi: 10.1016/S2352-3026(19)30031-6. Epub 2019 Mar 14.

Reference Type DERIVED
PMID: 30878319 (View on PubMed)

Provided Documents

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Document Type: Study Protocol and Statistical Analysis Plan

View Document

Other Identifiers

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P30CA006973

Identifier Type: NIH

Identifier Source: secondary_id

View Link

P01CA015396

Identifier Type: NIH

Identifier Source: secondary_id

View Link

NA_00002479

Identifier Type: OTHER

Identifier Source: secondary_id

J0676

Identifier Type: -

Identifier Source: org_study_id

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