Trial Outcomes & Findings for Efficacy and Safety Study for an Oral Contraceptive Containing Folate (NCT NCT00468481)

NCT ID: NCT00468481

Last Updated: 2014-04-23

Results Overview

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Recruitment status

COMPLETED

Study phase

PHASE3

Target enrollment

385 participants

Primary outcome timeframe

Week 24

Results posted on

2014-04-23

Participant Flow

572 subjects screened; 187 subjects screen failed; 385 subjects randomized

Participant milestones

Participant milestones
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Overall Study
STARTED
291
94
Overall Study
Subjects Treated
285
94
Overall Study
COMPLETED
203
70
Overall Study
NOT COMPLETED
88
24

Reasons for withdrawal

Reasons for withdrawal
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Overall Study
Adverse Event
13
3
Overall Study
Lost to Follow-up
25
8
Overall Study
Pregnancy
2
0
Overall Study
Protocol Violation
18
8
Overall Study
Withdrawal by Subject
12
3
Overall Study
susp/diag malignant/premalignant disease
1
0
Overall Study
Prohibited Medication
1
1
Overall Study
Egg Donor
1
0
Overall Study
Moved out of area
4
1
Overall Study
Non-Compliance
5
0
Overall Study
Other
6
0

Baseline Characteristics

Efficacy and Safety Study for an Oral Contraceptive Containing Folate

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=285 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=94 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Total
n=379 Participants
Total of all reporting groups
Age, Continuous
24.8 years
n=5 Participants
24.6 years
n=7 Participants
24.7 years
n=5 Participants
Sex: Female, Male
Female
285 Participants
n=5 Participants
94 Participants
n=7 Participants
379 Participants
n=5 Participants
Sex: Female, Male
Male
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants

PRIMARY outcome

Timeframe: Week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=124 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=45 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Red Blood Cell (RBC) Folate Level at 24 Weeks
1406.91 nmol/L
Interval 1354.13 to 1459.68
1022.21 nmol/L
Interval 934.6 to 1109.82

PRIMARY outcome

Timeframe: Week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=196 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Plasma Folate Level at 24 Weeks
60.55 nmol/L
Interval 58.13 to 62.97
41.67 nmol/L
Interval 37.5 to 45.85

SECONDARY outcome

Timeframe: Baseline and week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

The mean NTD risk reduction evaluated as the change from Baseline to Week 24 in NTD risk based on the formula of Daly et al (J Amer Med Assoc 1995;274(21):1698-702); NTD risk=exp (1.6463-1.2193 x natural log \[RBC folate\]) where natural log \[RBC folate\] is the natural log of RBC folate measured in nmol/L; Change from Baseline to Week 24 in NTD risk=NTD risk at Week 24 - NTD risk at Baseline

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=124 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=45 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Neural Tube Defect (NTD) Risk Reduction at Week 24
-0.51 per 1000 birth
Standard Deviation 0.41
-0.03 per 1000 birth
Standard Deviation 0.26

SECONDARY outcome

Timeframe: baseline and up to week 4

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=125 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=43 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Red Blood Cell (RBC) Folate Levels at Week 4
110.9 nmol/L
Standard Deviation 207.28
-37.6 nmol/L
Standard Deviation 195.88

SECONDARY outcome

Timeframe: baseline and up to week 8

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=122 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=43 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Red Blood Cell (RBC) Folate Levels at Week 8
310.3 nmol/L
Standard Deviation 231.47
68.2 nmol/L
Standard Deviation 155.26

SECONDARY outcome

Timeframe: baseline and up to week 12

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=127 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=41 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Red Blood Cell (RBC) Folate Levels at Week 12
405.8 nmol/L
Standard Deviation 243.88
86.5 nmol/L
Standard Deviation 177.81

SECONDARY outcome

Timeframe: baseline and up to week 16

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=120 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=46 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Red Blood Cell (RBC) Folate Levels at Week 16
448.9 nmol/L
Standard Deviation 280.37
68.0 nmol/L
Standard Deviation 186.44

SECONDARY outcome

Timeframe: baseline and up to week 20

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=123 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=44 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Red Blood Cell (RBC) Folate Levels at Week 20
452.7 nmol/L
Standard Deviation 284.64
64.6 nmol/L
Standard Deviation 194.89

SECONDARY outcome

Timeframe: baseline and up to week 4

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=192 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=65 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Folate Levels at Week 4
15.2 nmol/L
Standard Deviation 22.07
0.6 nmol/L
Standard Deviation 14.29

SECONDARY outcome

Timeframe: baseline and up to week 8

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=193 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Folate Levels at Week 8
18.4 µg/L
Standard Deviation 19.17
2.2 µg/L
Standard Deviation 14.63

SECONDARY outcome

Timeframe: baseline and up to week 12

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=194 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Folate Levels at Week 12
17.6 nmol/L
Standard Deviation 20.1
0.3 nmol/L
Standard Deviation 15.24

SECONDARY outcome

Timeframe: baseline and up to week 16

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=194 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=65 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Folate Levels at Week 16
17.6 nmol/L
Standard Deviation 12.43
2.4 nmol/L
Standard Deviation 16.44

SECONDARY outcome

Timeframe: baseline and up to week 20

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=193 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Folate Levels at Week 20
15.1 nmol/L
Standard Deviation 22.71
-1.6 nmol/L
Standard Deviation 15.65

SECONDARY outcome

Timeframe: baseline and up to week 4

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

Homocysteine concentrations in plasma were determined by Fluorescence Polarization Immunoassays (FPIA) using the Abbot AxSym analyzer in a clinical laboratory setting.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=194 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=65 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Homocysteine Levels at Week 4
0 µg/L
Standard Deviation 1.04
0 µg/L
Standard Deviation 0.69

SECONDARY outcome

Timeframe: baseline and up to week 8

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Homocysteine concentrations in plasma were determined by Fluorescence Polarization Immunoassays (FPIA) using the Abbot AxSym analyzer in a clinical laboratory setting.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=195 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Homocysteine Levels at Week 8
-0.2 µg/L
Standard Deviation 0.9
0 µg/L
Standard Deviation 1

SECONDARY outcome

Timeframe: baseline and up to week 12

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Homocysteine concentrations in plasma were determined by Fluorescence Polarization Immunoassays (FPIA) using the Abbot AxSym analyzer in a clinical laboratory setting.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=196 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Homocysteine Levels at Week 12
-0.3 µg/L
Standard Deviation 1.07
0 µg/L
Standard Deviation 0.93

SECONDARY outcome

Timeframe: baseline and up to week 16

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Homocysteine concentrations in plasma were determined by Fluorescence Polarization Immunoassays (FPIA) using the Abbot AxSym analyzer in a clinical laboratory setting.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=194 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=65 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Homocysteine Levels at Week 16
-0.2 µg/L
Standard Deviation 1.02
-0.1 µg/L
Standard Deviation 0.93

SECONDARY outcome

Timeframe: baseline and up to week 20

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Homocysteine concentrations in plasma were determined by Fluorescence Polarization Immunoassays (FPIA) using the Abbot AxSym analyzer in a clinical laboratory setting.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=195 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Homocysteine Levels at Week 20
-0.2 µg/L
Standard Deviation 1.07
-0.2 µg/L
Standard Deviation 0.91

SECONDARY outcome

Timeframe: baseline and up to week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Homocysteine concentrations in plasma were determined by Fluorescence Polarization Immunoassays (FPIA) using the Abbot AxSym analyzer in a clinical laboratory setting.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=195 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=66 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Change From Baseline in Plasma Homocysteine Levels at Week 24
-0.3 µg/L
Standard Deviation 0.93
0.1 µg/L
Standard Deviation 1.24

POST_HOC outcome

Timeframe: at baseline (week 0)

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=90 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=40 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Baseline
910.9 nmol/L
Standard Deviation 276.05
915.1 nmol/L
Standard Deviation 233.97

POST_HOC outcome

Timeframe: at baseline (week 0)

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=54 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=12 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Baseline
1122.8 nmol/L
Standard Deviation 504.7
1345.0 nmol/L
Standard Deviation 301.76

POST_HOC outcome

Timeframe: up to week 4

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=80 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=35 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 4
1007.1 nmol/L
Standard Deviation 329.67
889.5 nmol/L
Standard Deviation 262.42

POST_HOC outcome

Timeframe: up to week 4

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=50 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=9 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 4
1175.7 nmol/L
Standard Deviation 428.58
1256.1 nmol/L
Standard Deviation 190.89

POST_HOC outcome

Timeframe: up to week 8

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=76 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=33 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 8
1184.8 nmol/L
Standard Deviation 338.5
970.4 nmol/L
Standard Deviation 252.79

POST_HOC outcome

Timeframe: up to week 8

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=50 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=10 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 8
1412.7 nmol/L
Standard Deviation 436.69
1407.6 nmol/L
Standard Deviation 290.18

POST_HOC outcome

Timeframe: up to week 12

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=79 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=33 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 12
1308.9 nmol/L
Standard Deviation 374.61
1025.2 nmol/L
Standard Deviation 287.55

POST_HOC outcome

Timeframe: up to week 12

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=50 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=10 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 12
1469.8 nmol/L
Standard Deviation 454.62
1378.4 nmol/L
Standard Deviation 375.15

POST_HOC outcome

Timeframe: up to week 16

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=76 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=35 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 16
1365.2 nmol/L
Standard Deviation 403.12
967.6 nmol/L
Standard Deviation 255.78

POST_HOC outcome

Timeframe: up to week 16

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=50 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=11 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 16
1460.5 nmol/L
Standard Deviation 472.23
1454.7 nmol/L
Standard Deviation 355.5

POST_HOC outcome

Timeframe: up to week 20

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=78 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=33 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 20
1419.8 nmol/L
Standard Deviation 558.1
981.3 nmol/L
Standard Deviation 252.85

POST_HOC outcome

Timeframe: up to week 20

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=49 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=13 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 20
1486.6 nmol/L
Standard Deviation 462.8
1435.9 nmol/L
Standard Deviation 319.33

POST_HOC outcome

Timeframe: up to week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=87 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=37 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 24
1355.3 nmol/L
Standard Deviation 364.85
949.2 nmol/L
Standard Deviation 256.26

POST_HOC outcome

Timeframe: up to week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

RBC folate=(\[whole blood folate\*100\]-\[plasma folate\*(100-hematocrit)\])/hematocrit

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=51 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=10 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Red Blood Cell (RBC) Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 24
1500.3 nmol/L
Standard Deviation 525.71
1316.1 nmol/L
Standard Deviation 237.44

POST_HOC outcome

Timeframe: at baseline (week 0)

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=120 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=47 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Baseline
41.7 nmol/L
Standard Deviation 16.2
41.5 nmol/L
Standard Deviation 15.57

POST_HOC outcome

Timeframe: at baseline (week 0)

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=76 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=19 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Baseline
50.3 nmol/L
Standard Deviation 18.45
47.1 nmol/L
Standard Deviation 17.3

POST_HOC outcome

Timeframe: up to week 4

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=119 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=47 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 4
59.6 nmol/L
Standard Deviation 24.83
43.1 nmol/L
Standard Deviation 17.98

POST_HOC outcome

Timeframe: up to week 4

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=73 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=18 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 4
60.4 nmol/L
Standard Deviation 27.56
45.9 nmol/L
Standard Deviation 21.19

POST_HOC outcome

Timeframe: up to week 8

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=118 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=47 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 8
60.8 nmol/L
Standard Deviation 22.41
41.8 nmol/L
Standard Deviation 14.96

POST_HOC outcome

Timeframe: up to week 8

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=75 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=19 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 8
68.1 nmol/L
Standard Deviation 26.60
54.1 nmol/L
Standard Deviation 30.16

POST_HOC outcome

Timeframe: up to week 12

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=119 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=47 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 12
59.5 nmol/L
Standard Deviation 20.39
42.3 nmol/L
Standard Deviation 17.19

POST_HOC outcome

Timeframe: up to week 12

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=75 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=19 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 12
67.4 nmol/L
Standard Deviation 23.69
46.4 nmol/L
Standard Deviation 17.55

POST_HOC outcome

Timeframe: up to week 16

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=118 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=46 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 16
59.4 nmol/L
Standard Deviation 18.89
43.2 nmol/L
Standard Deviation 18.4

POST_HOC outcome

Timeframe: up to week 16

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=76 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=19 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 16
67.5 nmol/L
Standard Deviation 26.38
51.8 nmol/L
Standard Deviation 21.08

POST_HOC outcome

Timeframe: up to week 20

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=118 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=47 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 20
59.8 nmol/L
Standard Deviation 21.11
39.7 nmol/L
Standard Deviation 16.74

POST_HOC outcome

Timeframe: up to week 20

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=75 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=19 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 20
61.5 nmol/L
Standard Deviation 24.16
45.9 nmol/L
Standard Deviation 14.54

POST_HOC outcome

Timeframe: up to week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=120 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=47 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (Without Additional Folate Supplementation) at Week 24
58.7 nmol/L
Standard Deviation 17.73
39.8 nmol/L
Standard Deviation 18.02

POST_HOC outcome

Timeframe: up to week 24

Population: Analysis was based on Per Protocol Set (PPS) defined as all subjects who had no major protocol deviations and completed 24 weeks of treatment with valid data for one of the co-primary efficacy variables at baseline and week 24.

Folate concentrations in plasma were determined by an appropriately validated microbiological assay.

Outcome measures

Outcome measures
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=76 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=19 Participants
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Mean Plasma Folate Levels by Additional Folate Supplementation (With Additional Folate Supplementation) at Week 24
64.0 nmol/L
Standard Deviation 22.61
43.7 nmol/L
Standard Deviation 16.5

Adverse Events

Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)

Serious events: 3 serious events
Other events: 103 other events
Deaths: 0 deaths

Drospirenone (DRSP)/Ethinylestradiol (EE)

Serious events: 0 serious events
Other events: 44 other events
Deaths: 0 deaths

Serious adverse events

Serious adverse events
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=285 participants at risk
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=94 participants at risk
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Infections and infestations
Pneumonia
0.35%
1/285 • Number of events 1
0.00%
0/94
Investigations
Human papilloma virus test positive
0.35%
1/285 • Number of events 1
0.00%
0/94
Neoplasms benign, malignant and unspecified (incl cysts and polyps)
Cervix carcinoma stage 0
0.35%
1/285 • Number of events 1
0.00%
0/94
Reproductive system and breast disorders
Cervical dysplasia
0.35%
1/285 • Number of events 1
0.00%
0/94

Other adverse events

Other adverse events
Measure
Drospirenone (DRSP)/Ethinylestradiol (EE)/Metafolin (MTHF)
n=285 participants at risk
1 tablet 0.020 mg EE/3.0 mg DRSP/0.451 mg L-5-MTHF as calcium salt given orally/daily for 24 days followed by 1 tablet 0.451 mg L-5-MTHF as calcium salt given orally/daily for 4 days over a time period of 24 weeks
Drospirenone (DRSP)/Ethinylestradiol (EE)
n=94 participants at risk
1 tablet 0.020 mg EE/3.0 mg DRSP \[YAZ\] given orally/daily for 24 days followed by 1 placebo tablet given orally/daily for 4 days over a time period of 24 weeks
Gastrointestinal disorders
Abdominal pain
0.35%
1/285 • Number of events 1
3.2%
3/94 • Number of events 3
Infections and infestations
Bronchitis
2.1%
6/285 • Number of events 6
1.1%
1/94 • Number of events 1
Infections and infestations
Influenza
2.1%
6/285 • Number of events 6
3.2%
3/94 • Number of events 3
Infections and infestations
Nasopharyngitis
3.9%
11/285 • Number of events 11
2.1%
2/94 • Number of events 2
Infections and infestations
Pharyngitis
0.35%
1/285 • Number of events 1
2.1%
2/94 • Number of events 2
Infections and infestations
Sinusitis
3.2%
9/285 • Number of events 9
3.2%
3/94 • Number of events 3
Infections and infestations
Upper respiratory tract infection
10.5%
30/285 • Number of events 32
9.6%
9/94 • Number of events 10
Infections and infestations
Urinary tract infection
3.5%
10/285 • Number of events 11
2.1%
2/94 • Number of events 2
Investigations
Blood cholinesterase decreased
0.35%
1/285 • Number of events 1
2.1%
2/94 • Number of events 2
Investigations
Blood triglycerides increased
0.00%
0/285
2.1%
2/94 • Number of events 2
Investigations
Gamma-glutamyltransferase increased
0.70%
2/285 • Number of events 2
2.1%
2/94 • Number of events 2
Investigations
Low density lipoprotein increased
6.0%
17/285 • Number of events 17
8.5%
8/94 • Number of events 8
Investigations
Prothrombin level decreased
0.00%
0/285
2.1%
2/94 • Number of events 2
Investigations
Human papilloma virus test positive
3.5%
10/285 • Number of events 10
2.1%
2/94 • Number of events 2
Musculoskeletal and connective tissue disorders
Arthralgia
0.35%
1/285 • Number of events 1
2.1%
2/94 • Number of events 2
Nervous system disorders
Headache
2.1%
6/285 • Number of events 9
4.3%
4/94 • Number of events 6
Nervous system disorders
Migraine
0.70%
2/285 • Number of events 3
2.1%
2/94 • Number of events 2
Psychiatric disorders
Anxiety
0.35%
1/285 • Number of events 1
3.2%
3/94 • Number of events 3
Reproductive system and breast disorders
Cervical dysplasia
3.5%
10/285 • Number of events 10
4.3%
4/94 • Number of events 4
Respiratory, thoracic and mediastinal disorders
Cough
1.8%
5/285 • Number of events 5
5.3%
5/94 • Number of events 5
Respiratory, thoracic and mediastinal disorders
Sinus congestion
0.00%
0/285
2.1%
2/94 • Number of events 2
Respiratory, thoracic and mediastinal disorders
Oropharyngeal pain
0.00%
0/285
2.1%
2/94 • Number of events 2
Skin and subcutaneous tissue disorders
Rash
1.8%
5/285 • Number of events 5
5.3%
5/94 • Number of events 5

Additional Information

Therapeutic Area Head

BAYER

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place

Restriction type: LTE60