Trial Outcomes & Findings for A Phase 2 Study to Evaluate Immune Responses of FluMist® (NCT NCT00461981)

NCT ID: NCT00461981

Last Updated: 2021-10-06

Results Overview

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

101 participants

Primary outcome timeframe

Post Dose 1 (28 to 42 days post Dose 1)

Results posted on

2021-10-06

Participant Flow

A total of 101 subjects were entered and randomized into the study at 15 sites in the United States of America between 07 May 2007 and 28 Jun 2007.

Participant milestones

Participant milestones
Measure
FluMist, Influenza Virus Vaccine Live
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Overall Study
STARTED
50
51
Overall Study
COMPLETED
39
42
Overall Study
NOT COMPLETED
11
9

Reasons for withdrawal

Reasons for withdrawal
Measure
FluMist, Influenza Virus Vaccine Live
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Overall Study
Lost to Follow-up
9
6
Overall Study
Withdrawal by Subject
2
3

Baseline Characteristics

A Phase 2 Study to Evaluate Immune Responses of FluMist®

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
FluMist, Influenza Virus Vaccine Live
n=50 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=51 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Total
n=101 Participants
Total of all reporting groups
Age, Continuous
23.1 months
STANDARD_DEVIATION 7.0 • n=5 Participants
24.4 months
STANDARD_DEVIATION 7.0 • n=7 Participants
23.8 months
STANDARD_DEVIATION 7.0 • n=5 Participants
Age, Customized
12 to < 24 months
26 participants
n=5 Participants
24 participants
n=7 Participants
50 participants
n=5 Participants
Age, Customized
24 to < 36 months
24 participants
n=5 Participants
27 participants
n=7 Participants
51 participants
n=5 Participants
Sex: Female, Male
Female
22 Participants
n=5 Participants
21 Participants
n=7 Participants
43 Participants
n=5 Participants
Sex: Female, Male
Male
28 Participants
n=5 Participants
30 Participants
n=7 Participants
58 Participants
n=5 Participants
Race (NIH/OMB)
American Indian or Alaska Native
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Asian
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Native Hawaiian or Other Pacific Islander
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Race (NIH/OMB)
Black or African American
22 Participants
n=5 Participants
16 Participants
n=7 Participants
38 Participants
n=5 Participants
Race (NIH/OMB)
White
26 Participants
n=5 Participants
31 Participants
n=7 Participants
57 Participants
n=5 Participants
Race (NIH/OMB)
More than one race
2 Participants
n=5 Participants
4 Participants
n=7 Participants
6 Participants
n=5 Participants
Race (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Hispanic or Latino
6 Participants
n=5 Participants
9 Participants
n=7 Participants
15 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Not Hispanic or Latino
44 Participants
n=5 Participants
42 Participants
n=7 Participants
86 Participants
n=5 Participants
Ethnicity (NIH/OMB)
Unknown or Not Reported
0 Participants
n=5 Participants
0 Participants
n=7 Participants
0 Participants
n=5 Participants
Region of Enrollment
United States
50 participants
n=5 Participants
51 participants
n=7 Participants
101 participants
n=5 Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=44; n=39), and had a baseline HAI titer of 4 or less (n=24; n=27).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=24 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=27 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI)Seroconversion Following the First Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
20.8 Percentage of Participants
81.5 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=23; n=26), and had a baseline HAI titer of 4 or less (n=14; n=19).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=14 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=19 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the Second Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
28.6 Percentage of Participants
89.5 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=42; n=39), and had a baseline HAI titer of 4 or less (n=20; n=26).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /ca A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=20 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=26 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI)Seroconversion Following the First Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
75.0 Percentage of Participants
30.8 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=21; n=26), and had a baseline HAI titer of 4 or less (n=13; n=19).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /ca A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=13 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=19 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI)Seroconversion Following the Second Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
84.6 Percentage of Participants
68.4 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=43; n=40), and had a baseline HAI titer of 4 or less (n=34; n=30).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Wisconsin/67/05 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=34 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=30 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI)Seroconversion Following the First Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
61.8 Percentage of Participants
80.0 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=22; n=26), and had a baseline HAI titer of 4 or less (n=18; n=18).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Wisconsin/67/05 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=18 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=18 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the Second Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
94.4 Percentage of Participants
100 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=44; n=37), and had a baseline HAI titer of 4 or less (n=34; n=31).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the B /wt B/Malaysia/2506/04 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=34 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=31 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the First Dose - B /wt B/Malaysia/2506/04 Influenza Strain
64.7 Percentage of Participants
29.0 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=23; n=25), and had a baseline HAI titer of 4 or less (n=20; n=20).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the B /wt B/Malaysia/2506/04 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=20 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=20 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the Second Dose - B /wt B/Malaysia/2506/04 Influenza Strain
90.0 Percentage of Participants
95.0 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=43; n=39), and had a baseline HAI titer of 4 or less (n=24; n=29).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/Solomon Island/3/06 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=24 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=29 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the First Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
25.0 Percentage of Participants
10.3 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=22; n=26), and had a baseline HAI titer of 4 or less (n=14; n=21).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /wt A/Solomon Island/3/06 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=14 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=21 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the Second Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
71.4 Percentage of Participants
33.3 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=43; n=40), and had a baseline HAI titer of 4 or less (n=36; n=31).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Brisbane/10/2007 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=36 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=31 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the First Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
47.2 Percentage of Participants
35.5 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=22; n=26), and had a baseline HAI titer of 4 or less (n=19; n=19).

The percentage of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Brisbane/10/2007 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=19 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=19 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Hemagglutination Inhibition (HAI) Seroconversion Following the Second Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
78.9 Percentage of Participants
94.7 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=44; n=39), and had a baseline HAI titer of 4 or less (n=24; n=27).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=24 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=27 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
4.4 Titer
Interval 2.59 to 8.23
10.1 Titer
Interval 7.6 to 13.37

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=23; n=26), and had a baseline HAI titer of 4 or less (n=14; n=19).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2)for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=14 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=19 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
6.2 Titer
Interval 3.12 to 13.13
30.9 Titer
Interval 19.92 to 46.09

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=42; n=39), and had a baseline HAI titer of 4 or less (n=20; n=26).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /ca A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=20 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=26 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
19.0 Titer
Interval 10.56 to 34.3
5.7 Titer
Interval 3.69 to 9.39

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=21; n=26), and had a baseline HAI titer of 4 or less (n=13; n=19).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /ca A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=13 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=19 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
37.6 Titer
Interval 19.8 to 67.51
11.5 Titer
Interval 6.91 to 19.2

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=43; n=40), and had a baseline HAI titer of 4 or less (n=34; n=30).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Wisconsin/67/05 Influenza Strain antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=34 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=30 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
12.5 Titer
Interval 7.53 to 21.28
11.8 Titer
Interval 8.38 to 17.15

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=22; n=26), and had a baseline HAI titer of 4 or less (n=18; n=18).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Wisconsin/67/05 Influenza Strain antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=18 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=18 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
27.4 Titer
Interval 16.63 to 43.55
47.0 Titer
Interval 30.79 to 69.12

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=44; n=37), and had a baseline HAI titer of 4 or less (n=34; n=31).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the B /wt B/Malaysia/2506/04 Influenza Strain antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=34 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=31 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - B /wt B/Malaysia/2506/04 Influenza Strain
9.8 Titer
Interval 6.52 to 15.36
4.1 Titer
Interval 3.2 to 5.35

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=23; n=25), and had a baseline HAI titer of 4 or less (n=20; n=20).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the B /wt B/Malaysia/2506/04 Influenza Strain antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=20 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=20 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - B /wt B/Malaysia/2506/04 Influenza Strain
16.0 Titer
Interval 10.56 to 24.25
33.1 Titer
Interval 21.86 to 50.21

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=43; n=39), and had a baseline HAI titer of 4 or less (n=24; n=29)

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/Solomon Island/3/06 Influenza Strain antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=24 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=29 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
4.2 Titer
Interval 2.75 to 7.23
2.9 Titer
Interval 2.15 to 4.3

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=22; n=26), and had a baseline HAI titer of 4 or less (n=14; n=21).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /wt A/Solomon Island/3/06 Influenza Strain antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=14 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=21 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
8.0 Titer
Interval 4.42 to 14.49
4.6 Titer
Interval 3.17 to 6.78

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days post Dose 1 (n=44; n=40), no major protocol violations (n=43; n=40), and had a baseline HAI titer of 4 or less (n=36; n=31).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Brisbane/10/2007 Influenza Strain antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=36 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=31 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
7.0 Titer
Interval 4.49 to 11.1
4.7 Titer
Interval 3.27 to 7.0

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=23; n=26), no major protocol violations (n=22; n=26), and had a baseline HAI titer of 4 or less (n=19; n=19).

The HAI GMTs of baseline seronegative subjects (baseline titer of 4 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Brisbane/10/2007 Influenza Strain antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=19 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=19 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Hemagglutination Inhibition (HAI) Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
10.7 Titer
Interval 6.91 to 16.59
17.2 Titer
Interval 11.11 to 26.66

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=40; n=34), and had a baseline HAI titer of 10 or less (n=19; n=18).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=19 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=18 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the First Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
26.3 Percentage of Participants
72.2 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=21; n=19), and had a baseline HAI titer of 10 or less (n=12; n=12).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=12 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=12 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the Second Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
75.0 Percentage of Participants
100 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=40; n=35), and had a baseline HAI titer of 10 or less (n=20; n=20).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /ca A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=20 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=20 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the First Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
80.0 Percentage of Participants
15.0 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=20; n=22), and had a baseline HAI titer of 10 or less (n=12; n=13).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /ca A/New Caledonia/20/999 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=12 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=13 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the Second Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
91.7 Percentage of Participants
92.3 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=35; n=31), and had a baseline HAI titer of 10 or less (n=27; n=21).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Wisconsin/67/05 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=27 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=21 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the First Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
63.0 Percentage of Participants
100 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=17; n=22), and had a baseline HAI titer of 10 or less (n=12; n=16).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Wisconsin/67/05 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=12 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=16 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the Second Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
91.7 Percentage of Participants
100 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=30; n=27), and had a baseline HAI titer of 10 or less (n=22; n=21).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the B /wt B/Malaysia/2506/04 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=22 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=21 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the First Dose - B /wt B/Malaysia/2506/04 Influenza Strain
40.9 Percentage of Participants
19.0 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=16; n=16), and had a baseline HAI titer of 10 or less (n=13; n=12).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the B /wt B/Malaysia/2506/049 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=13 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=12 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Matched Strain-specific Microneutralization Seroconversion Following the Second Dose - B /wt B/Malaysia/2506/04 Influenza Strain
69.2 Percentage of Participants
91.7 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=16; n=14), and had a baseline HAI titer of 10 or less (n=1; n=3).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/Solomon Island/3/06 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=1 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=3 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Microneutralization Seroconversion Following the First Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
100 Percentage of Participants
66.7 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=7; n=8), and had a baseline HAI titer of 10 or less (n=1; n=2).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /wt A/Solomon Island/3/06 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=1 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=2 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Microneutralization Seroconversion Following the Second Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
100 Percentage of Participants
100 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=7; n=12), and had a baseline HAI titer of 10 or less (n=3; n=7).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Brisbane/10/2007 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=3 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=7 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Microneutralization Seroconversion Following the First Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
33.3 Percentage of Participants
42.9 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=7; n=8), and had a baseline HAI titer of 10 or less (n=5; n=5).

The percentage of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Brisbane/10/2007 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=5 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=5 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Percentage of Subjects With Antigenically Mismatched Strain-specific Microneutralization Seroconversion Following the Second Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
80.0 Percentage of Participants
100 Percentage of Participants

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=40; n=34), and had a baseline HAI titer of 10 or less (n=19; n=18).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=19 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=18 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
10.7 Titer
Interval 8.47 to 13.6
47.5 Titer
Interval 32.9 to 67.24

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=21; n=19), and had a baseline HAI titer of 10 or less (n=12; n=12).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /wt A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=12 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=12 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H1N1 /wt A/New Caledonia/20/99 Influenza Strain
24.5 Titer
Interval 15.87 to 37.75
226.2 Titer
Interval 126.95 to 391.6

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=40; n=35), and had a baseline HAI titer of 10 or less (n=20; n=20).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /ca A/New Caledonia/20/99 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=20 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=20 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
56.6 Titer
Interval 31.39 to 101.96
11.7 Titer
Interval 9.16 to 15.68

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=20; n=22), and had a baseline HAI titer of 10 or less (n=12; n=13).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /ca A/New Caledonia/20/999 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=12 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=13 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H1N1 /ca A/New Caledonia/20/99 Influenza Strain
164.4 Titer
Interval 79.63 to 339.03
49.6 Titer
Interval 33.3 to 68.39

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=35; n=31), and had a baseline HAI titer of 10 or less (n=27; n=21).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Wisconsin/67/05 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=27 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=21 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
83.1 Titer
Interval 37.46 to 177.29
61.4 Titer
Interval 45.64 to 82.68

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=17; n=22), and had a baseline HAI titer of 10 or less (n=12; n=16).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Wisconsin/67/05 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=12 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=16 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H3N2 /wt A/Wisconsin/67/05 Influenza Strain
190.1 Titer
Interval 75.41 to 415.1
433.3 Titer
Interval 287.1 to 653.93

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=30; n=27), and had a baseline HAI titer of 10 or less (n=22; n=21).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the B /wt B/Malaysia/2506/04 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=22 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=21 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - B /wt B/Malaysia/2506/04 Influenza Strain
21.9 Titer
Interval 12.24 to 41.82
8.9 Titer
Interval 6.72 to 12.39

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=16; n=14), and had a baseline HAI titer of 10 or less (n=1; n=3).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H1N1 /wt A/Solomon Island/3/06 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=1 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=3 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
28.0 Titer
Interval 28.0 to 28.0
31.5 Titer
Interval 28.0 to 40.0

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=16; n=16), and had a baseline HAI titer of 10 or less (n=13; n=12).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the B /wt B/Malaysia/2506/049 antigenically matched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=13 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=12 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - B /wt B/Malaysia/2506/04 Influenza Strain
34.0 Titer
Interval 18.39 to 71.81
67.1 Titer
Interval 42.12 to 105.14

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=7; n=8), and had a baseline HAI titer of 10 or less (n=1; n=2).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H1N1 /wt A/Solomon Island/3/06 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=1 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=2 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H1N1 /wt A/Solomon Island/3/06 Influenza Strain
160.0 Titer
Interval 160.0 to 160.0
40.0 Titer
Interval 40.0 to 40.0

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-42 days after Dose 1 (n=41; n=40), no major protocol violations (n=7; n=12), and had a baseline HAI titer of 10 or less (n=3; n=7).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 1 (28-42 days after Dose 1) for the H3N2 /wt A/Brisbane/10/2007 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=3 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=7 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the First Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
22.4 Titer
Interval 10.0 to 113.0
28.2 Titer
Interval 15.5 to 56.59

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: The immunogenicity (IM) population included all subjects who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had valid IM assay results obtained at baseline and at 28-35 days post Dose 2 (n=22; n=26), no major protocol violations (n=7; n=8), and had a baseline HAI titer of 10 or less (n=5; n=5).

The microneutralization GMTs of baseline seronegative subjects (baseline titer of 10 or less) achieving a 4 or more fold increase in titer from baseline at Post Dose 2 (28-35 days after Dose 2) for the H3N2 /wt A/Brisbane/10/2007 antigenically mismatched influenza strain

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=5 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=5 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Microneutralization Geometric Mean Titers (GMTs) in Baseline Seronegative Subjects Following the Second Dose - H3N2 /wt A/Brisbane/10/2007 Influenza Strain
56.3 Titer
Interval 24.38 to 121.2
85.8 Titer
Interval 52.85 to 129.87

PRIMARY outcome

Timeframe: Post Dose 1 (7 to 10 days post Dose 1)

Population: Evaluable subjects for the ELISPOT immunogenicity included those who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had baseline data (n=30; n=35), and any protocol-specified post-dose timepoint data for B-cell ELISPOT (n=16; n=17).

Counts of antibody secreting cells (ASCs) per 10\^6 peripheral blood mononuclear cells (PBMCs) for influenza-specific response (ie, anti-IgG fluorescein \[FLU\] or anti-IgA FLU) and influenza-specific response after adjusting plate background response (ie, anti-IgG FLU/anti-IgG total or anti-IgA FLU/anti-IgA total) as measured by B-cell ELISPOT assay at 7 to 10 days after Dose 1

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=16 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=17 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgG FLU
6.5 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 574.5
57.0 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 556.5
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgG FLU/Anti-IgG Total
0.030 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 0.796
0.204 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 2.2
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgA FLU
4.8 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 49.5
22.0 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 82.0
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgA FLU/Anti-IgA Total
0.011 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 0.161
0.043 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 0.3

PRIMARY outcome

Timeframe: Post Dose 2 (7 to 10 days post Dose 2)

Population: Evaluable subjects for the ELISPOT immunogenicity included those who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV), had baseline data (n=30; n=35), and any protocol-specified post-dose timepoint data for B-cell ELISPOT (n=10; n=9).

Counts of antibody secreting cells (ASCs) per 10\^6 peripheral blood mononuclear cells (PBMCs) for influenza-specific response (ie, anti-IgG fluorescein \[FLU\] or anti-IgA FLU) and influenza-specific response after adjusting plate background response (ie, anti-IgG FLU/anti-IgG total or anti-IgA FLU/anti-IgA total) as measured by B-cell ELISPOT assay at 7 to 10 days after Dose 2

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=10 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=9 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgG FLU
45.5 Counts of ASCs per 10^6 PBMCs
Interval 13.0 to 212.0
45.0 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 350.0
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgG FLU/Anti-IgG Total
0.161 Counts of ASCs per 10^6 PBMCs
Interval 0.017 to 0.53
0.076 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 2.0
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgA FLU
7.0 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 53.0
7.0 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 33.0
Immunogenicity Response by B-cell IgG and IgA ELISPOT Assay
Anti-IgA FLU/Anti-IgA Total
0.015 Counts of ASCs per 10^6 PBMCs
Interval 0.003 to 0.094
0.018 Counts of ASCs per 10^6 PBMCs
Interval 0.0 to 0.075

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Population: Evaluable subjects for the ELISPOT immunogenicity included those who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV) and had valid pre-Dose 1 and post-Dose 1 T-cell Elispot results (n=12; n=15)

Median number of PBMCs secreting interferon-gamma as measured by the number of spot forming cells per 200,000 PBMCs (SPC/2 x 10\^5 PBMCs) as measured by the T-cell Elispot assay at 28 to 42 days after Dose 1. The results were summarized for wild-type (wt) influenza-specific response (wt fluorescein \[FLU\]) after adjusting plate background response at 28 to 42 days after Dose 1

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=12 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=15 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Median Fold-Rises in the Number of Interferon-gamma Elispots Per 200,000 Peripheral Blood Mononuclear Cells (PBMCs) by T-cell Elispot Assay Following the First Dose
3.3 SPC/2 x 10^5 PBMCs
Interval 0.3 to 6.0
1.2 SPC/2 x 10^5 PBMCs
Interval 0.1 to 155.1

PRIMARY outcome

Timeframe: Post Dose 2 (28 to 35 days post Dose 2)

Population: Evaluable subjects for the ELISPOT immunogenicity included those who received at least 1 full dose of study vaccine (n=50 for FluMist; n=51 for TIV) and had valid pre-Dose 1 and any post-Dose T-cell Elispot results (n=3; n=6)

Median number of PBMCs secreting interferon-gamma as measured by the number of spot forming cells per 200,000 PBMCs (SPC/2 x 10\^5 PBMCs) as measured by the T-cell Elispot assay at 28 to 35 days after Dose 2. The results were summarized for wild-type (wt) influenza-specific response (wt fluorescein \[FLU\]) after adjusting plate background response at 28 to 35 days after Dose 2

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=3 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=6 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Median Fold-Rises in the Number of Interferon-gamma Elispots Per 200,000 Peripheral Blood Mononuclear Cells (PBMCs) by T-cell Elispot Assay
3.6 SPC/2 x 10^5 PBMCs
Interval 0.8 to 4.8
3.2 SPC/2 x 10^5 PBMCs
Interval 2.2 to 14.6

PRIMARY outcome

Timeframe: Post Dose 1 (28 to 42 days post Dose 1)

Distribution of IFN-alpha/beta gene signature scores at 7 to 10 days after Dose 1. IFN alpha/beta gene signature scores were calculated as the average fold change in a panel of 21 type 1 IFN-inducible genes. The distribution of IFN alpha/beta gene signature scores ranged from -4 to 4, with -4 representing the lowest level of activity and 4 representing the highest level of activity. The percentage of subjects by IFN-alpha/beta gene signature score for each treatment group were compared.

Outcome measures

Outcome measures
Measure
FluMist, Influenza Virus Vaccine Live
n=43 Participants
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=42 Participants
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of 4 (%)
0.09 Units on a scale
0.00 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of -1 (%)
0.07 Units on a scale
0.29 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of -4 (%)
0.02 Units on a scale
0.00 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of -3 (%)
0.00 Units on a scale
0.00 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of -2 (%)
0.02 Units on a scale
0.02 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of 0 (%)
0.23 Units on a scale
0.57 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of 1 (%)
0.21 Units on a scale
0.07 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of 2 (%)
0.21 Units on a scale
0.05 Units on a scale
Distribution of Interferon (IFN)-Alpha/Beta Gene Signature Scores Among All Subjects
Gene signature score of 3 (%)
0.14 Units on a scale
0.00 Units on a scale

Adverse Events

FluMist, Influenza Virus Vaccine Live

Serious events: 0 serious events
Other events: 26 other events
Deaths: 0 deaths

TIV, Trivalent Inactivated Influenza Virus Vaccine

Serious events: 0 serious events
Other events: 31 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
FluMist, Influenza Virus Vaccine Live
n=50 participants at risk
FluMist, Influenza Virus Vaccine Live, Intranasal, 0.25 mL will be administered intranasally for each of two doses
TIV, Trivalent Inactivated Influenza Virus Vaccine
n=51 participants at risk
TIV, Trivalent Inactivated Influenza Virus Vaccine, Intramuscular, 0.25 mL will be administered intramuscularly for each of two doses
Injury, poisoning and procedural complications
Arthropod Bite
6.0%
3/50 • Number of events 3 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
2.0%
1/51 • Number of events 1 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Gastrointestinal disorders
Constipation
0.00%
0/50 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
3.9%
2/51 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Respiratory, thoracic and mediastinal disorders
Cough
8.0%
4/50 • Number of events 4 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
9.8%
5/51 • Number of events 5 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Skin and subcutaneous tissue disorders
Dermatitis Diaper
0.00%
0/50 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
3.9%
2/51 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Gastrointestinal disorders
Diarrhoea
10.0%
5/50 • Number of events 5 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
2.0%
1/51 • Number of events 1 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Respiratory, thoracic and mediastinal disorders
Epistaxis
4.0%
2/50 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
0.00%
0/51 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
General disorders
Irritability
4.0%
2/50 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
2.0%
1/51 • Number of events 1 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Respiratory, thoracic and mediastinal disorders
Nasal Congestion
4.0%
2/50 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
2.0%
1/51 • Number of events 1 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Infections and infestations
Nasopharyngitis
4.0%
2/50 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
3.9%
2/51 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
General disorders
Pyrexia
8.0%
4/50 • Number of events 4 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
21.6%
11/51 • Number of events 11 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Respiratory, thoracic and mediastinal disorders
Rhinorrhoea
22.0%
11/50 • Number of events 11 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
23.5%
12/51 • Number of events 12 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Gastrointestinal disorders
Teething
2.0%
1/50 • Number of events 1 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
7.8%
4/51 • Number of events 4 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Infections and infestations
Upper Respiratory Tract Infection
4.0%
2/50 • Number of events 2 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
2.0%
1/51 • Number of events 1 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
Gastrointestinal disorders
Vomiting
6.0%
3/50 • Number of events 3 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.
5.9%
3/51 • Number of events 3 • Adverse events (AEs) were collected from the time of administration of the first dose of study vaccine (Study Day 0) through 42 days after administration of the final dose.
Legal representatives were given worksheets to capture AEs. The study staff contacted the subject's legal representative at the scheduled clinic visits and 28 to 35 days post Dose 2 to collect AEs. A telephone contact occurred at 43 to 57 days after administration of the final dose of study vaccine to collect AEs through 42 days.

Additional Information

Christopher Ambrose, MD

MedImmune, LLC

Phone: 301-398-0000

Results disclosure agreements

  • Principal investigator is a sponsor employee MedImmune has 60 days to review results communications prior to public release and may delete information that compromises ongoing studies or is considered proprietary. This restriction is not intended to compromise the objective scientific integrity of the manuscript, it being understood that results shall be published regardless of outcome.
  • Publication restrictions are in place

Restriction type: OTHER