Temsirolimus and Bevacizumab in Treating Patients With Stage III or Stage IV Malignant Melanoma

NCT ID: NCT00397982

Last Updated: 2017-06-09

Basic Information

• Recruitment Status: COMPLETED
• Clinical Phase: PHASE2
• Total Enrollment: 17 participants
• Study Classification: INTERVENTIONAL
• Study Start Date: 2008-01-31
• Study Completion Date: 2013-07-31

Brief Summary

This phase II trial is studying how well giving temsirolimus together with bevacizumab works in treating patients with stage III or stage IV malignant melanoma. Temsirolimus may stop the growth of tumor cells by blocking some of the enzymes needed for their growth. Monoclonal antibodies, such as bevacizumab, can block tumor growth in different ways. Some block the ability of tumor cells to grow and spread. Others find tumor cells and help kill them or carry tumor-killing substances to them. Bevacizumab may also stop the growth of malignant melanoma by blocking blood flow to the tumor. Giving temsirolimus together with bevacizumab may kill more tumor cells.

Detailed Description

PRIMARY OBJECTIVES:

I. Determine the objective tumor response rate (complete response and partial response) in patients with stage III or IV melanoma treated with temsirolimus and bevacizumab.

SECONDARY OBJECTIVES:

I. Describe the adverse event profile of this regimen in these patients. II. Determine the efficacy of this regimen, in terms of progression-free survival, in these patients.

III. Compare pre- vs post-treatment measurements of biomarkers and vascular system/immune system parameters in patients treated with this regimen.

IV. Correlate tumor and blood biomarkers with clinical response in these patients.

OUTLINE: This is a multicenter study.

Patients receive temsirolimus intravenously (IV) over 30 minutes on days 1 and 8 and bevacizumab IV over 30-90 minutes on day 8. Treatment repeats every 14 days for a maximum of 26 courses in the absence of disease progression or unacceptable toxicity. Patients undergo tumor resection on day 9 of course 2.Blood samples are collected during courses 1 and 2. Samples are examined by flow cytometry to evaluate peripheral blood mononuclear cells for molecular effects of study agents. Patients also undergo normal and tumor tissue biopsy (by core needle biopsy, incisional biopsy, or surgical resection) during courses 1 and 2. Samples are examined by immunohistochemistry, western blotting, protein array technology, gene expression analyses, DNA mutation analyses, and genomic analyses for pre-and post-treatment measurements of target molecules (epidermal growth factor receptor, B-Raf, MEK, MAPK), downstream pathway components (PI-3 kinase, AKT, mTOR), markers of angiogenesis, proliferation and apoptosis, markers that may modulate cell signaling or the response to investigational agents, and vascular and immune system parameters.

After completion of study treatment, patients are followed at 1 month, every 3 months for up to 2 years, and then periodically for up to 5 years.

Conditions

Recurrent Melanoma; Stage IIIB Skin Melanoma; Stage IIIC Skin Melanoma; Stage IV Skin Melanoma

Study Design

• Allocation Method: NA
• Intervention Model: SINGLE_GROUP
• Primary Study Purpose: TREATMENT
• Blinding Strategy: NONE

Study Groups

Treatment (enzyme inhibitor, monoclonal antibody)

Patients receive temsirolimus IV over 30 minutes on days 1 and 8 and bevacizumab IV over 30-90 minutes on day 8. Treatment repeats every 14 days for a maximum of 26 courses in the absence of disease progression or unacceptable toxicity. Patients undergo tumor resection on day 9 of course 2.

Group Type: EXPERIMENTAL

Bevacizumab

Intervention Type: BIOLOGICAL

Given IV

Laboratory Biomarker Analysis

Intervention Type: OTHER

Correlative studies

Temsirolimus

Intervention Type: DRUG

Given IV

Therapeutic Conventional Surgery

Intervention Type: PROCEDURE

Undergo tumor resection

Interventions

Bevacizumab

Given IV

Intervention Type: BIOLOGICAL

Laboratory Biomarker Analysis

Correlative studies

Intervention Type: OTHER

Temsirolimus

Given IV

Intervention Type: DRUG

Therapeutic Conventional Surgery

Undergo tumor resection

Intervention Type: PROCEDURE

Other Intervention Names

Anti-VEGF; Anti-VEGF Humanized Monoclonal Antibody; Anti-VEGF rhuMAb; Avastin; Bevacizumab Biosimilar BEVZ92; Bevacizumab Biosimilar BI 695502; Immunoglobulin G1 (Human-Mouse Monoclonal rhuMab-VEGF Gamma-Chain Anti-Human Vascular Endothelial Growth Factor), Disulfide With Human-Mouse Monoclonal rhuMab-VEGF Light Chain, Dimer; Recombinant Humanized Anti-VEGF Monoclonal Antibody; rhuMab-VEGF; CCI-779; CCI-779 Rapamycin Analog; Cell Cycle Inhibitor 779; Rapamycin Analog; Rapamycin Analog CCI-779; Torisel

Eligibility Criteria

Inclusion Criteria

• Histologically or cytologically confirmed melanoma

• Stage III or IV disease

• Recurrent disease allowed
• Measurable disease defined as ≥ 1 lesion that can be accurately measured in ≥ 1 dimension as ≥ 20 mm with conventional techniques OR ≥ 10 mm with spiral CT scan

• Tumor lesions in previously irradiated areas are not considered measurable disease
• Prior brain metastases allowed provided all of the following criteria are met:

• No more than a total of 5 brain metastases
• All metastases are no more than 2.5 cm
• Surgically resected or have been treated with gamma-knife or stereotactic radiosurgery
• More than 30 days since prior disease progression
• More than 30 days since prior steroids for managing brain metastases

• Concurrent steroids for other reasons allowed provided the dose is < that required for managing brain metastases
• Disease accessible for core needle biopsy, incisional biopsy, and/or surgical resection and meets one of the following criteria:

• One large tumor deposit ≥ 5 cm³ from which biopsies can be harvested multiple times
• Multiple deposits that can be biopsied or excised individually on different dates, measured as follows:

• One lesion ≥ 5 cm^3
• Two lesions ≥ 3 cm^3
• Three lesions ≥ 2 cm^3
• ECOG performance status 0-1
• Weight ≥ 110 pounds (without clothes)
• WBC ≥ 3,000 mm³
• Absolute neutrophil count ≥ 1,500/mm³
• Platelet count ≥ 100,000/mm³
• Bilirubin normal
• AST and ALT ≤ 2.5 times upper limit of normal
• Creatinine normal OR creatinine clearance ≥ 60 mL/min
• Urine protein: creatinine ratio < 1.0 OR 24-hour urine protein < 1,000 mg
• Fasting cholesterol < 350 mg/dL (cholesterol medications are allowed)
• Fasting triglycerides < 400 mg/dL
• PT INR ≤ 1.5 (unless on full-dose anticoagulants)
• Hematocrit < 41% (for males) or < 38% (for females)
• None of the following within the past 4 weeks:

• Uncontrolled intercurrent illness
• Ongoing or active acute (CTCAE v.3 grade 3 or 4) infection
• Abdominal fistula
• Gastrointestinal perforation
• Intra-abdominal abscess
• Serious or nonhealing wound, ulcer, or bone fracture
• No psychiatric illness or social situations that would preclude study compliance
• No clinically significant cardiovascular disease, including the following:

• Cerebrovascular accident within the past 6 months
• Transient ischemic attack within the past 6 months
• Myocardial ischemia within the past 6 months
• Myocardial infarction within the past 6 months
• Other thromboembolic event within the past 6 months
• Unstable angina within the past 6 months
• Uncontrolled hypertension (i.e., hypertension despite maximal therapy)
• New York Heart Association class II-IV heart disease
• Congestive heart failure
• Serious cardiac arrhythmia requiring medication
• Clinically significant peripheral vascular disease
• History of stroke
• Artificial valve, pacemaker, or similar device
• No uncontrolled diabetes

• Hemoglobin A1c < 7%
• No significant traumatic injury within the past 28 days
• No history of allergic reactions to compounds of similar chemical or biological composition to temsirolimus or bevacizumab
• No hypersensitivity to Chinese hamster ovary cell products or other recombinant human antibodies (e.g., infliximab)
• Not pregnant or nursing
• Negative pregnancy test
• Fertile patients must use effective contraception during and for ≥ 6 months after completion of study treatment
• HIV negative
• Hepatitis C negative
• See Disease Characteristics
• More than 4 weeks since any of the following prior treatments and recovered:

• Chemotherapy (6 weeks for nitrosoureas or mitomycin C)
• Radiotherapy to nontarget lesions or lesions that are not to be biopsied
• Immunotherapy
• Cytokine therapy
• Enzyme-inducing antiepileptic drugs (EIAEDs) or other CYP3A4 inducers
• Investigational agents
• More than 4 weeks since prior major surgery or open biopsy and recovered
• No prior temsirolimus, rapamycin, bevacizumab, or systemic therapies targeted primarily to vascular endothelial growth factor (VEGF), VEGF receptors, or to mTOR inhibition
• Concurrent full-dose anticoagulants (e.g., warfarin/low molecular weight heparin) with PT INR > 1.5 are allowed provided the following criteria are met:

• In-range INR (usually between 2 and 3.5) on a stable dose of oral anticoagulant or on a stable dose of low molecular weight heparin
• No active, clinically significant bleeding or pathological condition that carries a high risk of bleeding (e.g., tumor involving major vessels or known varices)

• Minimal tumor bleeding of the skin allowed at the clinician's discretion
• No concurrent medications or substances known to affect or with the potential to affect the activity or pharmacokinetics of the following:

• Temsirolimus
• Bevacizumab
• CYP450 isoenzymes
• No concurrent nonstudy-related surgical procedures
• No other concurrent anticancer agents or therapies

Exclusion Criteria

• Participants who have received these medications or treatments at any time ≤ 4 weeks of registration:

• Chemotherapy
• Radiotherapy to non-target lesions and lesions that are not to be biopsied. Prior radiotherapy to target lesions or lesions to be biopsied/resected is not permitted
• Immunotherapy
• Cytokine therapy
• Investigational reagents
• Invasive procedures defined as follows:

• Major surgical procedure, open biopsy or significant traumatic injury
• Anticipation of need for non-study related surgical procedures from registration until Cycle 26 (One year).
• Enzyme-inducing antiepileptic drugs (EIAEDs) or any other CYP3A4 inducer (Appendix C).

OR Participants who have not recovered from adverse events resulting from the administration of these agents/procedures > 4 weeks prior to registration.

• Participants who have received nitrosureas or mitomycin C ≤ 6 weeks of registration.
• History of allergic reactions attributed to compounds of similar chemical or biologic composition to CCI-779 or bevacizumab, or with known hypersensitivity to Chinese hamster ovary cell products (t-PA) or other recombinant human antibodies (e.g., Remicade®).
• Participants who have previously received CCI-779, rapamycin, bevacizumab, or systemic therapies targeted primarily to VEGF, VEGF receptors, or to mTOR inhibition.
• Participants who have experienced any of the following ≤ 4 weeks prior to registration:

• Uncontrolled intercurrent illness
• Infection, CTCAE3 grade 3 or 4 (either ongoing, chronic, or active infection)
• Abdominal fistula
• Gastrointestinal perforation
• Intra-abdominal abscess
• Serious or non-healing wound
• Serious or non-healing ulcer
• Serious or non-healing bone fracture.
• Potential subjects with clinically significant cardiovascular disease

• Recent history (defined as ≤ 6 months of registration) of thromboembolic events including cerebrovascular accident (CVA), transient ischemic attack (TIA), myocardial ischemia, myocardial infarction (MI)
• Uncontrolled hypertension (hypertension despite maximal therapy)
• Unstable angina ≤ 6 months of registration
• New York Heart Association Classification of ≥ class II heart disease
• Congestive heart failure
• Serious cardiac arrhythmia requiring medication
• Clinically significant peripheral vascular disease
• Have a history of stroke
• Have an artificial valve, pace-maker, or similar device
• Psychiatric illness/social situations that would limit compliance with study requirements.
• Pregnant (positive pregnancy test) or nursing women. Both fertile men and women must agree to use adequate contraceptive measures during study therapy and for at least 6 months after the completion of their participation in the study therapy.
• PT INR > 1.5, (unless the potential subject is on full dose anticoagulants and meet criteria described in Section 3.1.8).
• Participants with uncontrolled diabetes, defined as having a HGBA1C ≥ 7%.

• Minimum Eligible Age: 18 Years
• Eligible Sex: ALL
• Accepts Healthy Volunteers: No

Sponsors

Fox Chase Cancer Center

OTHER

Sponsor Role: collaborator

National Cancer Institute (NCI)

NIH

Sponsor Role: lead

Responsible Party

Responsibility Role: SPONSOR

Principal Investigators

Craig Slingluff

Role: PRINCIPAL_INVESTIGATOR

University of Virginia

Locations

Fox Chase Cancer Center

Philadelphia, Pennsylvania, United States

University of Virginia Cancer Center

Charlottesville, Virginia, United States

Countries

United States

References

Slingluff CL Jr, Petroni GR, Molhoek KR, Brautigan DL, Chianese-Bullock KA, Shada AL, Smolkin ME, Olson WC, Gaucher A, Chase CM, Grosh WW, Weiss GR, Wagenseller AG, Olszanski AJ, Martin L, Shea SM, Erdag G, Ram P, Gershenwald JE, Weber MJ. Clinical activity and safety of combination therapy with temsirolimus and bevacizumab for advanced melanoma: a phase II trial (CTEP 7190/Mel47). Clin Cancer Res. 2013 Jul 1;19(13):3611-20. doi: 10.1158/1078-0432.CCR-12-3919. Epub 2013 Apr 25.

Reference Type: RESULT

PMID: 23620404 (View on PubMed)

Wagenseller AG, Shada A, D'Auria KM, Murphy C, Sun D, Molhoek KR, Papin JA, Dutta A, Slingluff CL Jr. MicroRNAs induced in melanoma treated with combination targeted therapy of Temsirolimus and Bevacizumab. J Transl Med. 2013 Sep 18;11:218. doi: 10.1186/1479-5876-11-218.

Reference Type: RESULT

PMID: 24047116 (View on PubMed)

Other Identifiers

NCI-2009-00133

Identifier Type: REGISTRY

Identifier Source: secondary_id

NCI-7190

Identifier Type: -

Identifier Source: secondary_id

CDR0000512836

Identifier Type: -

Identifier Source: secondary_id

UVACC-MEL-47

Identifier Type: -

Identifier Source: secondary_id

MEL47

Identifier Type: OTHER

Identifier Source: secondary_id

7190

Identifier Type: OTHER

Identifier Source: secondary_id

P30CA044579

Identifier Type: NIH

Identifier Source: secondary_id

View Link

R21CA128367

Identifier Type: NIH

Identifier Source: secondary_id

View Link

NCI-2009-00133

Identifier Type: -

Identifier Source: org_study_id