Effect of Neoadjuvant or Adjuvant Systemic Therapy on Breast Cancers, Bone Marrow Cancer Cells, and Circulating Cancer Cells

NCT ID: NCT00353483

Last Updated: 2025-11-28

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

RECRUITING

Total Enrollment

300 participants

Study Classification

OBSERVATIONAL

Study Start Date

2005-09-14

Study Completion Date

2026-08-31

Brief Summary

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The main purpose of this study is to compare genetic markers present on tumor cells before and after chemotherapy.

Detailed Description

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In this study, the investigators propose that persistent disseminated tumor cells (DTC) present after systemic therapy represent a unique subpopulation of all DTC, are predictors of a poor response to systemic therapy and correlate with poor clinical outcome. The investigators hypothesize that systemic therapy-resistant DTC can be identified by their expression of a unique constellation of tumor marker proteins which may be similar to those expressed by breast cancer stem cells. In this research, the investigators' specific aims are : 1) to characterize tumor markers expressed by DTC which are present after systemic therapy, 2) to compare the expression of these markers to that on DTC detected prior to systemic therapy, 3) to correlate expression of the defined tumor markers on DTC with clinical outcome of breast cancer patients to identify those markers that are predictive of disease recurrence, 4) to utilize biomarkers identified in Specific Aims 1 and 2 to isolate purified DTC for further molecular analysis.

Conditions

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Breast Neoplasms

Study Design

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Observational Model Type

COHORT

Study Time Perspective

PROSPECTIVE

Study Groups

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Tissue, blood, and bone marrow (optional) collection

* Undergo neoadjuvant systemic therapy

* initial surgery for sentinel lymph node biopsy/portacath placement
* definitive cancer surgery (if applicable)
* when portacath is removed (1 year, if available)
* if metastatic disease develops or is present in accessible sites, a sample may be collected at the time of specimen collection for diagnosis
* Undergo Adjuvant Systemic Therapy

* initial surgery for a sentinel lymph node biopsy/portacath placement
* when portacath is removed (1 year, if available)
* If metastatic disease develops or is present in accessible sites, a sample may be collected at the time of specimen collection for diagnosis.
* Undergone neoadjuvant systemic therapy

* during definitive cancer surgery/portacath removal (if available)
* if metastatic disease develops or is present in accessible sites, a sample may be collected at the time of specimen collection for diagnosis

Peripheral blood draw

Intervention Type PROCEDURE

Breast tissue collection

Intervention Type PROCEDURE

Bone marrow biopsy

Intervention Type PROCEDURE

Interventions

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Peripheral blood draw

Intervention Type PROCEDURE

Breast tissue collection

Intervention Type PROCEDURE

Bone marrow biopsy

Intervention Type PROCEDURE

Eligibility Criteria

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Inclusion Criteria

* Recently diagnosed with clinical stage II, III, or IV breast cancer
* Planning to undergo neoadjuvant or adjuvant systemic therapy; patients who have already completed neoadjuvant systemic therapy are also eligible
* Must be \>= 18 years of age
* If female, must not be pregnant
* Must not have Hepatitis B, C, or HIV
* Must be willing and able to sign informed consent document
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Washington University School of Medicine

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Rebecca Aft, M.D., Ph.D.

Role: PRINCIPAL_INVESTIGATOR

Washington University School of Medicine

Locations

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Washington University School of Medicine

St Louis, Missouri, United States

Site Status RECRUITING

Countries

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United States

Central Contacts

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Rebecca Aft, MD, PhD

Role: CONTACT

314-747-0063

Facility Contacts

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Rebecca Aft, MD, PhD

Role: primary

314-747-0063

Tracey Guthrie

Role: backup

314-747-4404

References

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Diel IJ, Cote RJ. Bone marrow and lymph node assessment for minimal residual disease in patients with breast cancer. Cancer Treat Rev. 2000 Feb;26(1):53-65. doi: 10.1053/ctrv.1999.0150.

Reference Type BACKGROUND
PMID: 10660491 (View on PubMed)

Braun S, Pantel K, Muller P, Janni W, Hepp F, Kentenich CR, Gastroph S, Wischnik A, Dimpfl T, Kindermann G, Riethmuller G, Schlimok G. Cytokeratin-positive cells in the bone marrow and survival of patients with stage I, II, or III breast cancer. N Engl J Med. 2000 Feb 24;342(8):525-33. doi: 10.1056/NEJM200002243420801.

Reference Type BACKGROUND
PMID: 10684910 (View on PubMed)

Braun S, Naume B. Circulating and disseminated tumor cells. J Clin Oncol. 2005 Mar 10;23(8):1623-6. doi: 10.1200/JCO.2005.10.073. No abstract available.

Reference Type BACKGROUND
PMID: 15755968 (View on PubMed)

Janni W, Rack B, Schindlbeck C, Strobl B, Rjosk D, Braun S, Sommer H, Pantel K, Gerber B, Friese K. The persistence of isolated tumor cells in bone marrow from patients with breast carcinoma predicts an increased risk for recurrence. Cancer. 2005 Mar 1;103(5):884-91. doi: 10.1002/cncr.20834.

Reference Type BACKGROUND
PMID: 15666325 (View on PubMed)

Klein CA, Blankenstein TJ, Schmidt-Kittler O, Petronio M, Polzer B, Stoecklein NH, Riethmuller G. Genetic heterogeneity of single disseminated tumour cells in minimal residual cancer. Lancet. 2002 Aug 31;360(9334):683-9. doi: 10.1016/S0140-6736(02)09838-0.

Reference Type BACKGROUND
PMID: 12241875 (View on PubMed)

Choesmel V, Pierga JY, Nos C, Vincent-Salomon A, Sigal-Zafrani B, Thiery JP, Blin N. Enrichment methods to detect bone marrow micrometastases in breast carcinoma patients: clinical relevance. Breast Cancer Res. 2004;6(5):R556-70. doi: 10.1186/bcr898. Epub 2004 Jul 29.

Reference Type BACKGROUND
PMID: 15318937 (View on PubMed)

Lesurf R, Griffith OL, Griffith M, Hundal J, Trani L, Watson MA, Aft R, Ellis MJ, Ota D, Suman VJ, Meric-Bernstam F, Leitch AM, Boughey JC, Unzeitig G, Buzdar AU, Hunt KK, Mardis ER. Genomic characterization of HER2-positive breast cancer and response to neoadjuvant trastuzumab and chemotherapy-results from the ACOSOG Z1041 (Alliance) trial. Ann Oncol. 2017 May 1;28(5):1070-1077. doi: 10.1093/annonc/mdx048.

Reference Type DERIVED
PMID: 28453704 (View on PubMed)

Related Links

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http://www.siteman.wustl.edu

Alvin J. Siteman Cancer Center at Barnes-Jewish Hospital and Washington University School of Medicine

Other Identifiers

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05-0648 / 201101961

Identifier Type: -

Identifier Source: org_study_id

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