Donor Stem Cell Transplant in Treating Young Patients With Myelodysplastic Syndrome, Leukemia, Bone Marrow Failure Syndrome, or Severe Immunodeficiency Disease

NCT ID: NCT00295971

Last Updated: 2012-11-12

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

21 participants

Study Classification

INTERVENTIONAL

Study Start Date

2005-04-30

Study Completion Date

2011-12-31

Brief Summary

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RATIONALE: Giving chemotherapy and total body irradiation before a donor bone marrow transplant or peripheral blood stem cell transplant helps stop the growth of cancer cells. It also helps stop the patient's immune system from rejecting the donor's stem cells. When the healthy stem cells from a donor are infused into the patient they may help the patient's bone marrow make stem cells, red blood cells, white blood cells, and platelets. Sometimes the transplanted cells from a donor can make an immune response against the body's normal cells. Giving antithymocyte globulin and removing the T cells from the donor cells before transplant may stop this from happening.

PURPOSE: This phase I trial is studying the side effects and best dose of donor T cells and antithymocyte globulin when given together with chemotherapy and total-body irradiation in treating young patients who are undergoing T-cell depleted donor stem cell transplant for myelodysplastic syndrome, leukemia, bone marrow failure syndrome, or severe immunodeficiency disease.

Detailed Description

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OBJECTIVES:

* Determine the efficacy and toxicity of stem cell-enriched, T-cell-depleted, haplocompatible allogeneic hematopoietic stem cell transplantation in children with high-risk myelodysplastic syndromes, high-risk leukemia, severe acquired or congenital cytopenias, or primary immunodeficiency diseases.
* Determine the toxicity of a fludarabine and thiotepa-containing regimen in combination with lower doses of antithymocyte globulin in these patients.
* Determine the engraftment rate in patients treated with this regimen.
* Define T-cell reconstitution in these patients.
* Determine the toxicity and effects of administering stem cell and T-cell boosts after transplantation on hematopoiesis and immune reconstitution in these patients.

OUTLINE: This is a dose-escalation study of donor CD3+ cells and antithymocyte globulin (ATG).

* Cytoreductive regimen: Patients undergo total body irradiation twice daily on days -9 to -7. Patients also receive fludarabine IV on days -6 to -2, thiotepa IV every 12 hours on day -6, and ATG IV on days -5 to -2.
* Transplantation: Patients undergo CD34-enriched, T-cell-depleted, haplocompatible allogeneic peripheral blood stem cell or bone marrow transplantation on day 0.
* Donor T-cell infusion:Patients with no active graft-vs-host disease and evidence of engraftment but low absolute CD34+ lymphocyte count at 12 weeks post transplant may receive donor CD3+ cells at 4-week intervals.
* Donor stem cell boost: Patients with engraftment but either cytokine or transfusion dependent at 12 weeks post transplant may receive a boost of donor CD34+ cells.

Cohorts of 3-6 patients receive escalating doses of donor CD3+ cells and ATG until the optimum is determined. The optimum dose is defined as the dose at which both engraftment and T-cell recovery occur, without dose-limiting toxicity, in ≥ 5 of 6 patients.

After the completion of study treatment, patients are followed periodically for 5 years and then every 5 years thereafter.

PROJECTED ACCRUAL: A total of 21 patients will be accrued for this study.

Conditions

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Congenital Amegakaryocytic Thrombocytopenia Leukemia Myelodysplastic Syndromes Severe Congenital Neutropenia

Keywords

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childhood acute lymphoblastic leukemia in remission childhood acute myeloid leukemia in remission de novo myelodysplastic syndromes previously treated myelodysplastic syndromes secondary myelodysplastic syndromes secondary acute myeloid leukemia juvenile myelomonocytic leukemia chronic phase chronic myelogenous leukemia refractory anemia with excess blasts in transformation refractory anemia with excess blasts severe congenital neutropenia congenital amegakaryocytic thrombocytopenia childhood chronic myelogenous leukemia childhood myelodysplastic syndromes

Study Design

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Allocation Method

NA

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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Single arm of transplant

Receiving haplocompatible T cell depleted peripheral blood stem cell transplant

Group Type EXPERIMENTAL

anti-thymocyte globulin

Intervention Type BIOLOGICAL

therapeutic allogeneic lymphocytes

Intervention Type BIOLOGICAL

fludarabine phosphate

Intervention Type DRUG

thiotepa

Intervention Type DRUG

allogeneic bone marrow transplantation

Intervention Type PROCEDURE

allogeneic hematopoietic stem cell transplantation

Intervention Type PROCEDURE

in vitro-treated peripheral blood stem cell transplantation

Intervention Type PROCEDURE

total-body irradiation

Intervention Type RADIATION

Interventions

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anti-thymocyte globulin

Intervention Type BIOLOGICAL

therapeutic allogeneic lymphocytes

Intervention Type BIOLOGICAL

fludarabine phosphate

Intervention Type DRUG

thiotepa

Intervention Type DRUG

allogeneic bone marrow transplantation

Intervention Type PROCEDURE

allogeneic hematopoietic stem cell transplantation

Intervention Type PROCEDURE

in vitro-treated peripheral blood stem cell transplantation

Intervention Type PROCEDURE

total-body irradiation

Intervention Type RADIATION

Eligibility Criteria

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Inclusion Criteria

DISEASE CHARACTERISTICS:

* Diagnosis of one of the following:

* Acute lymphoblastic leukemia in ≥ 2nd remission or delayed remission induction
* High-risk myelodysplastic syndromes

* Refractory anemia with excess blasts (RAEB)
* RAEB in transformation
* Chronic myelogenous leukemia in second chronic phase

* No accelerated phase (\> 5% blasts in marrow)
* Juvenile myelomonocytic leukemia
* Acute nonlymphoblastic leukemia in \> 1st remission or induction failure and \< 30% blasts in marrow
* Severe aplastic anemia, defined as absolute neutrophil count \< 500/mm\^3 and platelet and/or red blood cell transfusion dependent

* Unresponsive to immunosuppressive therapy
* No Fanconi's anemia
* Congenital marrow aplasias unresponsive to cytokines and transfusion dependent
* Inherited immunodeficiency disease involving neutrophils or lymphocytes, including any of the following:

* Chediak-Higashi disease
* Wiskott-Aldrich syndrome
* Combined immunodeficiency disease (Nezelof's)
* Hyper IgM syndrome
* No relapsed disease
* Haplocompatible related donor, including parent, cousin, aunt, uncle, grandparent, half-sibling, or sibling (≥ 12 years of age), available

* 2 or 3 HLA antigen mismatch
* At least a 3 HLA antigen genotypic match
* No closely matched related or unrelated donor available in sufficient time to do the transplant

PATIENT CHARACTERISTICS:

* No active hepatitis or cytomegalovirus infection
* Cardiac ejection fraction ≥ 30%
* Creatinine clearance ≥ 70 mL/min
* DLCO ≥ 70% of predicted
* No active infection
* No HIV positivity

PRIOR CONCURRENT THERAPY:

* See Disease Characteristics
Minimum Eligible Age

1 Year

Maximum Eligible Age

17 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Cancer Institute (NCI)

NIH

Sponsor Role collaborator

University of California, San Francisco

OTHER

Sponsor Role lead

Responsible Party

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Morton Cowan

Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Morton J. Cowan, MD

Role: STUDY_CHAIR

University of California, San Francisco

Locations

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UCSF Helen Diller Family Comprehensive Cancer Center

San Francisco, California, United States

Site Status

Lineberger Comprehensive Cancer Center at University of North Carolina - Chapel Hill

Chapel Hill, North Carolina, United States

Site Status

Countries

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United States

Other Identifiers

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UCSF-01151

Identifier Type: -

Identifier Source: secondary_id

UCSF-H411-17122-07

Identifier Type: -

Identifier Source: secondary_id

CDR0000462168

Identifier Type: -

Identifier Source: org_study_id