Study of Intra-articular Delivery of tgAAC94 in Inflammatory Arthritis Subjects

NCT ID: NCT00126724

Last Updated: 2009-07-29

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

120 participants

Study Classification

INTERVENTIONAL

Study Start Date

2005-08-31

Study Completion Date

2009-05-31

Brief Summary

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The 13G01 clinical trial is a Phase I/II dose escalation study designed to be conducted in adults with inflammatory arthritis who have persistent moderate or severe swelling in one or more joints, without a disease severe enough to warrant a change in regimen for the next three months.

The study will permit subjects who are concurrently on anti-tumor necrosis factor (TNF)-alpha antagonists. For subjects on disease modifying antirheumatic drugs (DMARDs), a stable regimen for inflammatory arthritis for the previous three months, with no changes in doses in the four weeks prior to screening will be required.

The primary objectives are:

1. to evaluate the safety of intra-articular administration of tgAAC94 in subjects currently taking TNF-alpha antagonists, and
2. to evaluate the safety of repeat intra-articular administration of tgAAC94 (gene therapy vector).

Detailed Description

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tgAAC94 is a recombinant adeno-associated virus serotype 2 (AAV2) vector genetically engineered to contain the cDNA for a human tumor necrosis factor receptor (TNFR)-immunoglobulin (IgG1) Fc fusion (TNFR:Fc) gene. The DNA sequence of TNFR:Fc in tgAAC94 codes for a protein sequence identical to etanercept (Enbrel®). TNF-alpha has been strongly implicated as a major participant in the inflammatory cascade that leads to joint damage and destruction in diseases such as rheumatoid arthritis (RA), psoriatic arthritis (PsA) and ankylosing spondylitis (AS).

Intra-articular delivery of the TNFR:Fc gene (tgAAC94) should result in expression of the secreted protein in the joint space and provide local high concentrations of soluble TNFR:Fc for an extended period of time without requiring frequent administration. Thus, this proposed therapy would be useful in those inflammatory arthritis patients who have a persistently problematic joint despite the use of systemic TNF-alpha blockade or who have a limited number of arthritic joints.

Extensive preclinical studies using rAAV2 containing several different transgenes in a variety of animal models have shown efficient and persistent gene transfer and expression with minimal toxicity. The parent virus (wild-type AAV2) is a naturally occurring, non-replicating virus that depends on a helper virus, such as adenovirus, for replication. The recombinant AAV2 vector is unable to replicate in target host cells because it lacks the AAV genes, whose protein products are also required in trans, for replication and packaging of progeny virus. Extensive epidemiological studies have found AAV2 to be non-pathogenic.

Although there is no cure for arthritis, treatment has been revolutionized by the advent of anti-TNF-alpha therapies. These include etanercept (Enbrel®), infliximab (Remicade®) and adalimumab (Humira®), which consist of soluble TNF receptors, chimeric human-mouse anti-TNF-alpha monoclonal antibodies and fully human anti-TNF-alpha monoclonal antibodies, respectively. Clinical studies have shown these products to improve the signs and symptoms, inhibit the structural damage, and impact functional outcomes in patients with these inflammatory arthritides.

Conditions

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Arthritis, Rheumatoid Arthritis, Psoriatic Ankylosing Spondylitis

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

QUADRUPLE

Participants Caregivers Investigators Outcome Assessors

Study Groups

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1

1x10\^11 DRP/mL tgAAC94

Group Type ACTIVE_COMPARATOR

tgAAC94 gene therapy vector

Intervention Type GENETIC

Single Dose 1x10\^11 DRP/mL

tgAAC94 gene therapy vector

Intervention Type GENETIC

Second dose of 1x10\^11 DRP/mL tgAAC94 administered once target joint reaches predetermined criteria for re-injection (on or after Week 12) at the same concentration as initial dose.

2

1x10\^12 DRP/mL tgAAC94

Group Type ACTIVE_COMPARATOR

tgAAC94 gene therapy vector

Intervention Type GENETIC

Single Dose 1x10\^12 DRP/mL tgAAC94

tgAAC94 gene therapy vector

Intervention Type GENETIC

Second dose of 1x10\^12 DRP/mL tgAAC94 administered once target joint reaches predetermined criteria for re-injection (on or after Week 12) at the same concentration as initial dose.

3

1x10\^13 DRP/mL tgAAC94

Group Type ACTIVE_COMPARATOR

tgAAC94 gene therapy vector

Intervention Type GENETIC

Single Dose 1x10\^13 DRP/mL tgAAC94

tgAAC94 gene therapy vector

Intervention Type GENETIC

Second dose of 1x10\^13 DRP/mL tgAAC94 administered once target joint reaches predetermined criteria for re-injection (on or after Week 12) at the same concentration as initial dose.

4

Group Type PLACEBO_COMPARATOR

tgAAC94 placebo

Intervention Type GENETIC

placebo

Interventions

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tgAAC94 gene therapy vector

Single Dose 1x10\^11 DRP/mL

Intervention Type GENETIC

tgAAC94 gene therapy vector

Second dose of 1x10\^11 DRP/mL tgAAC94 administered once target joint reaches predetermined criteria for re-injection (on or after Week 12) at the same concentration as initial dose.

Intervention Type GENETIC

tgAAC94 placebo

placebo

Intervention Type GENETIC

tgAAC94 gene therapy vector

Single Dose 1x10\^12 DRP/mL tgAAC94

Intervention Type GENETIC

tgAAC94 gene therapy vector

Second dose of 1x10\^12 DRP/mL tgAAC94 administered once target joint reaches predetermined criteria for re-injection (on or after Week 12) at the same concentration as initial dose.

Intervention Type GENETIC

tgAAC94 gene therapy vector

Single Dose 1x10\^13 DRP/mL tgAAC94

Intervention Type GENETIC

tgAAC94 gene therapy vector

Second dose of 1x10\^13 DRP/mL tgAAC94 administered once target joint reaches predetermined criteria for re-injection (on or after Week 12) at the same concentration as initial dose.

Intervention Type GENETIC

Eligibility Criteria

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Inclusion Criteria

* Rheumatoid arthritis (RA), psoriatic arthritis (PsA), or ankylosing spondylitis (AS) diagnosed according to established criteria.
* Persistent moderate (grade 2) or severe (grade 3) swelling due to inflammatory arthritis in at least one peripheral joint eligible for injection.
* For subjects with RA, an adequate trial of at least one disease-modifying drug (DMARD) prior to screening.
* For subjects currently on DMARD(s), a stable regimen of inflammatory arthritis for the previous three months, with no changes in doses four weeks prior to screening.
* Age greater than 18 years and less than 75 years at the time of screening.
* Willingness to practice effective birth control measures during the study (through week 36), if male or female of reproductive ability.
* Able to give written informed consent.

Exclusion Criteria

* Disease severe enough to warrant a change in regimen for inflammatory arthritis in the next three months.
* Discontinuation of etanercept in the past because of safety concerns.
* Current use of anakinra (Kineret®)or abatacept (Orencia®).
* Corticosteroid therapy at doses higher than the equivalent of 10 mg prednisone per day.
* Steroid or hyaluronate injection in the target joint or receipt of an investigational agent less than four weeks prior to screening.
* Class IV ACR functional status (Hochberg et al., 1992).
* Any of the following laboratory values: Hemoglobin \<8.5 gm/dL, white blood cell count \<3500 per mm cube, platelet \<100 K/uL, creatinine \>2 mg/dL, bilirubin \>2 mg/dL, AST or ALT \>2 times the upper limit of normal, or abnormal coagulation profiles (\>2 seconds beyond upper range of normal PT or PTT).
* Known HIV infection, known hepatitis C infection, or known positive serologic test for hepatitis B surface antigen.
* Positive PPD, unless previously treated with appropriate prophylaxis.
* Pregnancy or lactation, either at the time of screening or planned in the next 18 months.
* Inflammatory bowel disease, such as Crohn's disease or ulcerative colitis.
* Serious medical disease, such as severe liver or kidney disease, uncompensated congestive heart failure, myocardial infarction within six months, unstable angina, uncontrolled hypertension, severe pulmonary disease or uncontrolled asthma, demyelinating neurological disease, history of cancer (other than cutaneous basal and squamous cell carcinoma) with less than five years documentation of a disease-free state, insulin-dependent diabetes, recurrent opportunistic infections or other concurrent medical condition that, in the opinion of the investigator, would make the subject unsuitable for the study.
* Unlikely to comply with protocol.
Minimum Eligible Age

18 Years

Maximum Eligible Age

75 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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Targeted Genetics Corporation

INDUSTRY

Sponsor Role lead

Responsible Party

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Targeted Genetics Corporation

Principal Investigators

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Alison Heald, MD

Role: STUDY_DIRECTOR

Targeted Genetics Corporation

Locations

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Sun Valley Arthritis Center

Glendale, Arizona, United States

Site Status

Catalina Pointe Clinical Research, Inc

Tuscon, Arizona, United States

Site Status

Desert Medical Advances

Palm Desert, California, United States

Site Status

Boling Clinical Trials

Upland, California, United States

Site Status

Denver Arthritis Research Center

Denver, Colorado, United States

Site Status

RASF-Clinical Research Center

Boca Raton, Florida, United States

Site Status

Ocala Rheumatology Research Center

Ocala, Florida, United States

Site Status

Radiant Research Stuart

Stuart, Florida, United States

Site Status

Coeur d'Alene Arthritis Clinic

Coeur d'Alene, Idaho, United States

Site Status

Northwestern Center for Clinical Research

Chicago, Illinois, United States

Site Status

The Arthritis Center

Springfield, Illinois, United States

Site Status

Arthritis and Osteoporosis Center of Maryland

Frederick, Maryland, United States

Site Status

Arthritis Center of Reno

Reno, Nevada, United States

Site Status

United Medical Associates

Johnson City, New York, United States

Site Status

Bone and Joint Hospital Research Dept.

Oklahoma City, Oklahoma, United States

Site Status

Altoona Center for Clinical Research

Duncansville, Pennsylvania, United States

Site Status

Rheumatic Disease Associates

Willow Grove, Pennsylvania, United States

Site Status

Austin Rheumatology Research

Austin, Texas, United States

Site Status

Arthritis Consultation Center

Dallas, Texas, United States

Site Status

Metroplex Clinical Research Center

Dallas, Texas, United States

Site Status

Radiant Research San Antonio Northeast

San Antonio, Texas, United States

Site Status

Seattle Rheumatology Associates, PLLC

Seattle, Washington, United States

Site Status

Countries

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United States

References

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Heald AE, Fudman EJ, Anklesaria P, Mease PJ; 13G01 Study Team. Single-joint outcome measures: preliminary validation of patient-reported outcomes and physical examination. J Rheumatol. 2010 May;37(5):1042-8. doi: 10.3899/jrheum.090827. Epub 2010 Mar 15.

Reference Type DERIVED
PMID: 20231202 (View on PubMed)

Frank KM, Hogarth DK, Miller JL, Mandal S, Mease PJ, Samulski RJ, Weisgerber GA, Hart J. Investigation of the cause of death in a gene-therapy trial. N Engl J Med. 2009 Jul 9;361(2):161-9. doi: 10.1056/NEJMoa0801066.

Reference Type DERIVED
PMID: 19587341 (View on PubMed)

Related Links

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Other Identifiers

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NIH Protocol Number: 0504-705

Identifier Type: -

Identifier Source: secondary_id

13G01

Identifier Type: -

Identifier Source: org_study_id

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