Trial Outcomes & Findings for AMD 3100 (Mozobil Plerixafor) to Mobilize Stem Cells for Donation (NCT NCT00075335)

NCT ID: NCT00075335

Last Updated: 2018-02-07

Results Overview

Examine G-CSF mobilization effect on cytokine polarization of T-cells. Analyze cytokine gene expression profiles using a Th1-Th2-Th3 RT-PCR plate in CD3+ T cells collected form subjects mobilized with 5 injections of G-CSF. 84 cytokine genes were analyzed for significant alteration in their profiles.

Recruitment status

COMPLETED

Study phase

PHASE2

Target enrollment

8 participants

Primary outcome timeframe

1 Day

Results posted on

2018-02-07

Participant Flow

Participant milestones

Participant milestones
Measure
AMD 3100 (Mozobil Plerixafor ) Volunteers
Peripheral blood hematopoietic progenitor cell mobilization with AMD 3100 (Mozobil plerixafor) in healthy volunteers. AMD 3100 volunteer response to peripheral blood stem cell mobilization
Overall Study
STARTED
8
Overall Study
COMPLETED
8
Overall Study
NOT COMPLETED
0

Reasons for withdrawal

Withdrawal data not reported

Baseline Characteristics

AMD 3100 (Mozobil Plerixafor) to Mobilize Stem Cells for Donation

Baseline characteristics by cohort

Baseline characteristics by cohort
Measure
AMD 3100 (Mozobil Plerixafor ) Volunteers
n=8 Participants
Peripheral blood hematopoietic progenitor cell mobilization with AMD 3100 (Mozobil plerixafor) in healthy volunteers
Age, Categorical
<=18 years
0 Participants
n=5 Participants
Age, Categorical
Between 18 and 65 years
8 Participants
n=5 Participants
Age, Categorical
>=65 years
0 Participants
n=5 Participants
Sex: Female, Male
Female
3 Participants
n=5 Participants
Sex: Female, Male
Male
5 Participants
n=5 Participants
Region of Enrollment
United States
8 participants
n=5 Participants

PRIMARY outcome

Timeframe: 1 Day

Population: 8 Subjects received G-CSF for 5 days.

Examine G-CSF mobilization effect on cytokine polarization of T-cells. Analyze cytokine gene expression profiles using a Th1-Th2-Th3 RT-PCR plate in CD3+ T cells collected form subjects mobilized with 5 injections of G-CSF. 84 cytokine genes were analyzed for significant alteration in their profiles.

Outcome measures

Outcome measures
Measure
AMD 3100 Volunteers
n=8 Participants
Peripheral blood hematopoietic progenitor cell mobilization with AMD 3100 (Mozobil plerixafor) in healthy volunteers
Change in Cytokine Gene Expression Profiles (84 Genes) in T Cells Following G-CSF Administration
16 unique cytokine genes affected

PRIMARY outcome

Timeframe: 1 Day

Population: Subjects who received plexirxafor 240mcg/kg. Data was collected 6 hours after injection.

Examine plerixafor mobilization effect on cytokine polarization of T-cells. Analyze cytokine gene expression profiles using a Th1-Th2-Th3 RT-PCR plate in CD3+ T cells collected form subjects mobilized with a single injection of plerixafor. 84 cytokine genes were analyzed for significant alteration in their profiles.

Outcome measures

Outcome measures
Measure
AMD 3100 Volunteers
n=8 Participants
Peripheral blood hematopoietic progenitor cell mobilization with AMD 3100 (Mozobil plerixafor) in healthy volunteers
Change in Cytokine Gene Expression Profiles (84 Genes) in T Cells Following Plerixafor Administration
0 unique cytokine genes affected

Adverse Events

AMD 3100 (Mozobil Plerixafor ) Volunteers

Serious events: 0 serious events
Other events: 8 other events
Deaths: 0 deaths

Serious adverse events

Adverse event data not reported

Other adverse events

Other adverse events
Measure
AMD 3100 (Mozobil Plerixafor ) Volunteers
n=8 participants at risk
Peripheral blood hematopoietic progenitor cell mobilization with AMD 3100 (Mozobil plerixafor) in healthy volunteers
Blood and lymphatic system disorders
elevated LDH
12.5%
1/8
Blood and lymphatic system disorders
elevated Lymphs (efficacy measure)
87.5%
7/8
Blood and lymphatic system disorders
elevated lymphs (expected)
12.5%
1/8
Blood and lymphatic system disorders
elevated Monos (expected)
100.0%
8/8
Blood and lymphatic system disorders
elevated Polys (expected)
100.0%
8/8
Blood and lymphatic system disorders
elevated WBCs (expected)
100.0%
8/8
Cardiac disorders
Sinus tachycardia (rate 103)
12.5%
1/8
Ear and labyrinth disorders
auditory (R ear) pain
12.5%
1/8
Gastrointestinal disorders
Cramps, abdominal
12.5%
1/8
Gastrointestinal disorders
feel need to vomit, no nausea
12.5%
1/8
General disorders
Headache
25.0%
2/8
General disorders
Hot feeling
12.5%
1/8
General disorders
Swelling, leg
12.5%
1/8
Immune system disorders
Injection site (erythema)
62.5%
5/8
Immune system disorders
Injection site (induration)
25.0%
2/8
Immune system disorders
Injection site (pain)
37.5%
3/8
Immune system disorders
Injection site (pruritus)
12.5%
1/8
Musculoskeletal and connective tissue disorders
muscle pain
12.5%
1/8
Nervous system disorders
paresthesia (facial tingling)
37.5%
3/8
Nervous system disorders
Syncope
25.0%
2/8

Additional Information

Dr. Richard Childs

NHLBI NIH

Phone: 301-594-8008

Results disclosure agreements

  • Principal investigator is a sponsor employee
  • Publication restrictions are in place