Conditioning Regimen for Allogeneic Hematopoietic Stem Cell Transplantation of Patients With Hematological Diseases

NCT ID: NCT00056966

Last Updated: 2012-06-29

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1/PHASE2

Total Enrollment

24 participants

Study Classification

INTERVENTIONAL

Study Start Date

2002-11-30

Study Completion Date

2006-12-31

Brief Summary

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Participants in this study have a hematologic malignancy (a disorder in the bone marrow that affects the body's ability to create blood) that might benefit from receiving an allogeneic stem cell transplant (meaning the cells come from a donor) from a family member or nearly identical matched donor. The donor may either be a matched sibling, a mismatched family member, or an unrelated person.

Usually these patients are given high doses of chemotherapy before receiving a stem cell transplant to keep their immune system from rejecting the donor stem cells and to kill any diseased cells that remain in the body. However, this group of patients have a high risk of developing possibly life-threatening treatment-related side effects such as infections, damage to vital organs such as lungs, liver, kidney and heart, as well as graft versus host disease (GVHD).

Instead of the high dose chemotherapy and radiotherapy usually given before a transplant, this research study uses a new pre-transplant combination of three drugs, Fludarabine, Anti-CD45 and CAMPATH-1H with low dose radiotherapy. Fludarabine is a chemotherapy drug while Anti-CD45 and CAMPATH-1H are antibodies against certain types of blood cells, including those which are causing this disease. CAMPATH-1H is particularly important because it stays active in the body for a long time after it is given, which means it may work longer to prevent GVHD symptoms. Anti-CD45 may help in eradicating residual malignant cells. All these agents also help in preventing rejection of donor stem cells. This study is designed to give a less intense chemotherapy and radiotherapy, so that the life-threatening toxicities of conventional high dose chemotherapy and radiotherapy regimen can be reduced, while maintaining the ability to cure cancer.

Detailed Description

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CAMPATH-1H will be given as a daily IV infusion for three days. Fludarabine will be given as a daily IV infusion for four days. Anti-CD45 will be given as a daily IV infusion for 4 days. Patients will then receive radiotherapy (also known as Total Body Irradiation or TBI) for one day. A summary of the treatment follows:

* Day - 8: CAMPATH-1H and Fludarabine
* Day - 7: CAMPATH-1H and Fludarabine
* Day - 6: CAMPATH-1H and Fludarabine
* Day - 5: Anti-CD45 and Fludarabine
* Day - 4: Anti-CD45
* Day - 3: Anti-CD45
* Day - 2: Anti-CD45
* Day - 1: TBI
* Day 0: Stem Cell Infusion (transplant)

To help prevent the body from developing GVHD, patients will also receive the drug FK506, starting two days before the transplant and continuing for at least one month.

Both the CAMPATH-1H and the Anti-CD45 can cause allergic reactions so patients will be given drugs to help prevent those reactions before receiving daily doses.

To see how CAMPATH-1H works in patients with hematologic malignancies, some patients will be asked to participate in pharmacokinetic studies. For this, approximately 13 blood samples will be taken from the central line scheduled before each infusion on Day -8 to Day -6, daily thereafter until Day 0, and then approximately once per week on days 7, 14, 21 and 28 post transplant. No more than 5 teaspoonfuls total will be drawn.

To see how Anti-CD45 works in patients with hematologic malignancies some patients will be asked to participate in pharmacokinetic studies. Approximately 22 blood samples will be taken from the central line scheduled before, during and after each infusion and after the end of the last infusion of Anti-CD45. No more than 10 teaspoonfuls total will be drawn over the course of the four anti-CD45 infusions.

Conditions

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Hematologic Malignancy

Keywords

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Acute myeloid leukemia Acute lymphoblastic leukemia Chronic myeloid leukemia Non-Hodgkin's lymphoma Hodgkin's disease Myelodysplastic syndrome Myeloproliferative disorders Multiple myeloma Severe aplastic anemia

Study Design

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Allocation Method

NON_RANDOMIZED

Intervention Model

PARALLEL

Primary Study Purpose

TREATMENT

Blinding Strategy

NONE

Study Groups

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1

recipients of HLA matched sibling transplants

Group Type EXPERIMENTAL

ANTI-CD45

Intervention Type DRUG

400ug/kg Day-5 through Day-2

CAMPATH-1H

Intervention Type DRUG

Day -8 through Day -6 Dosed per Institutional SOP

FK506

Intervention Type DRUG

Day -2 through Day 30 dose adjusted to maintain level between 5-15 ng/ml.

Fludarabine

Intervention Type DRUG

Day-8 through Day-5 30 mg/m2

Total Body Irradiation

Intervention Type RADIATION

Day-1 single dose 450 cGy

Stem cell infusion

Intervention Type PROCEDURE

Patients will receive peripheral blood stem cells from a HLA matched or one antigen mismatched related or unrelated donor (target CD34+ cell count \>2 x 106/kg). When peripheral stem cells are unavailable or insufficient, bone marrow (target mononuclear cell count \>2 x 108/kg) will be substituted.

2

recipients of unrelated or mismatched family donor transplants

Group Type EXPERIMENTAL

ANTI-CD45

Intervention Type DRUG

400ug/kg Day-5 through Day-2

CAMPATH-1H

Intervention Type DRUG

Day -8 through Day -6 Dosed per Institutional SOP

FK506

Intervention Type DRUG

Day -2 through Day 30 dose adjusted to maintain level between 5-15 ng/ml.

Fludarabine

Intervention Type DRUG

Day-8 through Day-5 30 mg/m2

Total Body Irradiation

Intervention Type RADIATION

Day-1 single dose 450 cGy

Stem cell infusion

Intervention Type PROCEDURE

Patients will receive peripheral blood stem cells from a HLA matched or one antigen mismatched related or unrelated donor (target CD34+ cell count \>2 x 106/kg). When peripheral stem cells are unavailable or insufficient, bone marrow (target mononuclear cell count \>2 x 108/kg) will be substituted.

Interventions

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ANTI-CD45

400ug/kg Day-5 through Day-2

Intervention Type DRUG

CAMPATH-1H

Day -8 through Day -6 Dosed per Institutional SOP

Intervention Type DRUG

FK506

Day -2 through Day 30 dose adjusted to maintain level between 5-15 ng/ml.

Intervention Type DRUG

Fludarabine

Day-8 through Day-5 30 mg/m2

Intervention Type DRUG

Total Body Irradiation

Day-1 single dose 450 cGy

Intervention Type RADIATION

Stem cell infusion

Patients will receive peripheral blood stem cells from a HLA matched or one antigen mismatched related or unrelated donor (target CD34+ cell count \>2 x 106/kg). When peripheral stem cells are unavailable or insufficient, bone marrow (target mononuclear cell count \>2 x 108/kg) will be substituted.

Intervention Type PROCEDURE

Other Intervention Names

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anti-CD52 alemtuzumab tacrolimus

Eligibility Criteria

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Inclusion Criteria

1. Patients with one of the following high risk diseases needing allogeneic hemopoietic stem cell transplantation:

Acute myeloid leukemia either a) Primary refractory, or b) Beyond first complete remission(CR1), or c) In CR1 with high risk of relapse

Acute lymphoblastic leukemia either a) Primary refractory, or b) Beyond first complete remission(CR1), or c) In CR1 with high risk of relapse

Chronic myeloid leukemia, either a) Accelerated phase, or b) Blast crisis, or c) Chronic phase and not achieving major cytogenetic response despite standard therapy

Chronic lymphocytic leukemia, either a) Primary refractory, or b) Beyond first complete remission(CR1),

Non Hodgkin's lymphoma, either a) Primary refractory, or b) Beyond first complete remission(CR1)

Hodgkin's disease, either a) Primary refractory, or b) Beyond first complete remission(CR1),

Myelodysplastic syndrome with IPSS score \> 0. (Appendix A)

Myeloproliferative disorders (with the exclusion of chronic myeloid leukemia) a) Primary Myelofibrosis with Lile score of 1 or 2 (Appendix B) b) Polycythemia Vera or Essential Thrombocythemia transformed to AML or Myelofibrosis and PV "spent phase"

Multiple Myeloma with stage II or III disease

Severe aplastic anemia
2. Conditions that increase Treatment Related Mortality (need one or more to be eligible):

Greater or equal to 35 years of age;

Ejection Fraction of less than 50%;

DLCO less than 50% or FEV1/FVC \< 80% of predicted value;

Diabetes Mellitus;

Renal insufficiency (serum creatinine abnormal);

Hepatic dysfunction-transaminases, or alkaline phosphatase, or bilirubin twice the upper limit of normal;

Prior recent history of systemic fungal infection;

Multiple prior treatment regimens (equal to or more than 3);

Significant Grade III or IV neurologic, cardiac, pulmonary, renal or hepatic toxicity from previous treatment;

Prior Autologous or Allogeneic Stem Cell transplantation;
3. Available Healthy Donor without any contraindications for donation. 5/6 or 6/6 related or unrelated donor (molecular typing for DRB1);
4. Patient and/or responsible person able to understand and sign consent

Exclusion Criteria

Pregnant and lactating women, or women unwilling to use contraception.

HIV positive patient

Unstable angina and uncompensated congestive heart failure (Zubrod of 3 or greater)

Severe chronic pulmonary disease requiring oxygen (Zubrod of 3 or greater)

Child's class C cirrhosis

Unstable cerebral vascular disease or recent hemorrhagic stroke (less than 6 months)

Patients with known allergy to rat serum products
Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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The Methodist Hospital Research Institute

OTHER

Sponsor Role collaborator

Center for Cell and Gene Therapy, Baylor College of Medicine

OTHER

Sponsor Role collaborator

Baylor College of Medicine

OTHER

Sponsor Role lead

Responsible Party

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George Carrum

Associate Professor

Responsibility Role PRINCIPAL_INVESTIGATOR

Principal Investigators

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Malcolm K Brenner, MD

Role: STUDY_CHAIR

Baylor College of Medicine

Locations

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Texas Children's Hospital

Houston, Texas, United States

Site Status

The Methodist Hospital

Houston, Texas, United States

Site Status

Countries

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United States

Other Identifiers

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ACHE

Identifier Type: -

Identifier Source: secondary_id

12472

Identifier Type: -

Identifier Source: org_study_id

NCT00602888

Identifier Type: -

Identifier Source: nct_alias