Bioengineered Skin and Wound Healing

NCT ID: NCT00007280

Last Updated: 2013-08-02

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE1

Total Enrollment

50 participants

Study Classification

INTERVENTIONAL

Study Start Date

2000-10-31

Study Completion Date

2005-08-31

Brief Summary

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This study will look at whether a graft of bioengineered skin (BSC), known commercially as Apligraf, stimulates the healing process in a person's own skin at the edge of a wound (known as the edge effect). The information from this study will provide a better understanding of the ways that grafts of bioengineered skin help the healing of chronic wounds.

We will assign study participants to either the bioengineered skin group or the control group. People in the control group will receive compression therapy with a multilayered compression bandage. We will examine each participant before starting treatment and then once a week for 24 weeks or until the wound heals. On the first day of treatment (day 0) and at week 3, week 6, and week 24 (end of treatment) we will take a small tissue sample from the wound for a biopsy. After the wound is completely healed, we will ask the patient to return once a month for 6 months to make sure the wound stays healed.

Detailed Description

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BSC is a two-layered sheet made from purified beef tendon collagen, living human cells, and a substance that maintains the cells until they are grafted (removed). We will obtain human cells from donors unrelated to the patient. The human cells have been tested for the presence of infectious agents and found to be free of disease-causing organisms such as hepatitis virus, the AIDS virus, bacteria, and fungi.

We will randomly assign (randomize) study participants to either the bioengineered skin group or control group (compression therapy with a multilayered compression bandage). Regardless of the group to which a patient is assigned, we expect participation in this study for 12 months following the start of study treatment. We will examine each patient at the screening visit (2 weeks before randomization) and then again 3-4 days before the start of treatment to make sure the wound is free of any signs of infection. After the initial randomization visit we will examine the wound once a week for 24 weeks or until the wound heals, whichever is earlier. As soon as we have determined that the wound is completely healed, we will ask the patient to return once a month for 6 months to make sure it remains healed.

Bioengineered skin group: We will apply BSC to the wound and cover it with xeroform dressing, foam bolster, gauze dressing, and compression bandage. If we do not note any improvement at the week 6 visit, we will apply BSC on the wound a second time.

Control group: We will place a multilayered compression bandage on the wound of participants assigned to this group.

Biopsies (small piece of skin tissue): At day 0 a biopsy will be taken from the thigh and leg ulcer. The biopsy from the thigh will require sutures and will be removed in ten days. Sometime between weeks 1 and 3, week 6, week 24 and week 48 (6 month follow-up) visits a biopsy will be taken from the ulcer (wound) if the ulcer has not healed. If the ulcer is healed at the week 48 visit, a light scraping of the healed wound will be performed.

Study examinations: All study examinations will include observation, measurement, and photography.

We can only admit women of childbearing age to the study if they are not breast feeding, not pregnant, or have been surgically sterilized or are using effective birth control. Because the effects of the proposed treatments on a fetus are unknown, we will remove from the study any woman who becomes pregnant while receiving BSC applications (day 0-week 3) and suggest another method of treatment.

Conditions

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Leg Ulcers

Keywords

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Bioengineered skin Venous leg ulcer

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

SINGLE_GROUP

Primary Study Purpose

TREATMENT

Blinding Strategy

DOUBLE

Participants Investigators

Interventions

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Bioengineered skin

Intervention Type DEVICE

Eligibility Criteria

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Inclusion Criteria

* Men or women at least 18 years old
* At least one ulcer (wound) greater than or equal to 2 centimeters
* Ulcer (wound) present for at least 3 months or greater
* Ankle/brachial index \> 0.7
* Patient must be ambulatory
* Patient must read, understand and sign informed consent

Exclusion Criteria

* Medical conditions limiting participation
* History of poor compliance, unreliability
* History of allergy to bovine collagen
* Gangrene, vasculitis, collagen vascular disease osteomyelitis or exposed tendons
* Use of systemic steroids/immunosuppressives
* History of diabetes mellitus
Minimum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)

NIH

Sponsor Role collaborator

Roger Williams Medical Center

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Vincent Falanga, MD

Role: PRINCIPAL_INVESTIGATOR

Roger Williams Medical Center

Locations

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Roger Williams Medical Center Dept. of Dermatology & Skin Surgery

Providence, Rhode Island, United States

Site Status

Countries

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United States

References

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Falanga V, Sabolinski M. A bilayered living skin construct (APLIGRAF) accelerates complete closure of hard-to-heal venous ulcers. Wound Repair Regen. 1999 Jul-Aug;7(4):201-7. doi: 10.1046/j.1524-475x.1999.00201.x.

Reference Type BACKGROUND
PMID: 10781211 (View on PubMed)

Falanga V, Isaacs C, Paquette D, Downing G, Kouttab N, Butmarc J, Badiavas E, Hardin-Young J. Wounding of bioengineered skin: cellular and molecular aspects after injury. J Invest Dermatol. 2002 Sep;119(3):653-60. doi: 10.1046/j.1523-1747.2002.01865.x.

Reference Type BACKGROUND
PMID: 12230509 (View on PubMed)

Nahm WK, Zhou L, Falanga V. Sustained ability for fibroblast outgrowth from stored neonatal foreskin: a model for studying mechanisms of fibroblast outgrowth. J Dermatol Sci. 2002 Feb;28(2):152-8. doi: 10.1016/s0923-1811(01)00157-8.

Reference Type BACKGROUND
PMID: 11858954 (View on PubMed)

Phillips TJ, Manzoor J, Rojas A, Isaacs C, Carson P, Sabolinski M, Young J, Falanga V. The longevity of a bilayered skin substitute after application to venous ulcers. Arch Dermatol. 2002 Aug;138(8):1079-81. doi: 10.1001/archderm.138.8.1079.

Reference Type BACKGROUND
PMID: 12164746 (View on PubMed)

Badiavas EV, Falanga V. Treatment of chronic wounds with bone marrow-derived cells. Arch Dermatol. 2003 Apr;139(4):510-6. doi: 10.1001/archderm.139.4.510.

Reference Type BACKGROUND
PMID: 12707099 (View on PubMed)

Kim BC, Kim HT, Park SH, Cha JS, Yufit T, Kim SJ, Falanga V. Fibroblasts from chronic wounds show altered TGF-beta-signaling and decreased TGF-beta Type II receptor expression. J Cell Physiol. 2003 Jun;195(3):331-6. doi: 10.1002/jcp.10301.

Reference Type BACKGROUND
PMID: 12704642 (View on PubMed)

Shen JT, Falanga V. Growth factors, signal transduction, and cellular responses. J Dermatol. 2003 Jan;30(1):5-16.

Reference Type BACKGROUND
PMID: 12598704 (View on PubMed)

Nahm WK, Philpot BD, Adams MM, Badiavas EV, Zhou LH, Butmarc J, Bear MF, Falanga V. Significance of N-methyl-D-aspartate (NMDA) receptor-mediated signaling in human keratinocytes. J Cell Physiol. 2004 Aug;200(2):309-17. doi: 10.1002/jcp.20010.

Reference Type BACKGROUND
PMID: 15174101 (View on PubMed)

Brem H, Kirsner RS, Falanga V. Protocol for the successful treatment of venous ulcers. Am J Surg. 2004 Jul;188(1A Suppl):1-8. doi: 10.1016/S0002-9610(03)00284-8.

Reference Type BACKGROUND
PMID: 15223495 (View on PubMed)

Saap LJ, Donohue K, Falanga V. Clinical classification of bioengineered skin use and its correlation with healing of diabetic and venous ulcers. Dermatol Surg. 2004 Aug;30(8):1095-100. doi: 10.1111/j.1524-4725.2004.30334.x.

Reference Type BACKGROUND
PMID: 15274699 (View on PubMed)

Butmarc J, Yufit T, Carson P, Falanga V. Human beta-defensin-2 expression is increased in chronic wounds. Wound Repair Regen. 2004 Jul-Aug;12(4):439-43. doi: 10.1111/j.1067-1927.2004.12405.x.

Reference Type BACKGROUND
PMID: 15260809 (View on PubMed)

Other Identifiers

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R01AR046557

Identifier Type: NIH

Identifier Source: secondary_id

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NIAMS-060

Identifier Type: -

Identifier Source: secondary_id

R01AR046557

Identifier Type: NIH

Identifier Source: org_study_id

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