A Blood Test to Look at Cells of the Immune System in Healthy Children

NCT ID: NCT00001109

Last Updated: 2008-07-30

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Total Enrollment

630 participants

Study Classification

OBSERVATIONAL

Brief Summary

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The purpose of this study is to learn more about some of the immune cells in the blood (CD4 cells, for example) of healthy children in order to better understand the differences in the blood cells of children infected with HIV.

Because children's bodies are still developing, their cells are different from those of adults, and their bodies respond differently to infections such as HIV. In order to understand how immune cells grow and mature so that they can fight HIV, it is important to see how these cells behave in normal children.

Detailed Description

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Early in life, the cells involved in immune selection differentiate into CD4+ and CD8+ cells. Currently there is insufficient information on the cell maturation and activation of these peripheral blood mononuclear cell (PBMC) subsets in normal children. The critical need for this information has been brought about by the pediatric HIV/AIDS epidemic, which requires the measurement of peripheral blood CD4+ T cells and other cells types for interpretation of HIV disease progression. The immunopathogenesis of pediatric HIV infection differs from that in adults but is not well understood. In order to better understand HIV disease progression in HIV-infected children, these PBMC subsets must be studied in normal children so that control values can be established.

Healthy infants, children, and adolescents presenting for routine care or elective surgery have a single blood sample obtained. Blood is used for complete blood count, peripheral blood mononuclear cell flow analysis for surface markers, and plasma and cell storage. Demographic information including age, sex, race, and ethnicity is obtained at the time of the blood draw. The reason for the patient's visit is also documented. No study drugs are administered or supplied as part of this study. Statistical analysis is used to estimate the median distribution of each CD4 and CD8 subset.

Conditions

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HIV Infections HIV Seronegativity

Keywords

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Monocytes Flow Cytometry HIV Seronegativity Phenotype Lymphocyte Subsets

Eligibility Criteria

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Inclusion Criteria

A child may be eligible for this study if he/she:

* Is 0 - 18 years old.
* Is HIV-negative and is born to an HIV-negative mother.
* Is healthy (no serious infection or disease).
* Has signed informed consent of a parent or legal guardian if under 18.

Exclusion Criteria

A child will not be eligible for this study if he/she:

* Is pregnant or breast-feeding.
* Has already completed this study once before.
* Is taking prescription medications other than prescription vitamins.
Minimum Eligible Age

1 Day

Maximum Eligible Age

18 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

Yes

Sponsors

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Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)

NIH

Sponsor Role collaborator

National Institute of Allergy and Infectious Diseases (NIAID)

NIH

Sponsor Role lead

Principal Investigators

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W Shearer

Role: STUDY_CHAIR

H Rosenblatt

Role: STUDY_CHAIR

Locations

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Univ of Alabama at Birmingham - Pediatric

Birmingham, Alabama, United States

Site Status

Univ of South Alabama

Mobile, Alabama, United States

Site Status

UCSD Med Ctr / Pediatrics / Clinical Sciences

La Jolla, California, United States

Site Status

Los Angeles County - USC Med Ctr

Los Angeles, California, United States

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Cedars Sinai / UCLA Med Ctr

Los Angeles, California, United States

Site Status

Harbor - UCLA Med Ctr / UCLA School of Medicine

Los Angeles, California, United States

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UCSF / Moffitt Hosp - Pediatric

San Francisco, California, United States

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Children's Hosp of Washington DC

Washington D.C., District of Columbia, United States

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Howard Univ Hosp

Washington D.C., District of Columbia, United States

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North Broward Hosp District

Fort Lauderdale, Florida, United States

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Univ of Florida Health Science Ctr / Pediatrics

Jacksonville, Florida, United States

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Univ of Miami (Pediatric)

Miami, Florida, United States

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Palm Beach County Health Dept

Riviera Beach, Florida, United States

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The Med Ctr Inc

Columbus, Georgia, United States

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Mt Sinai Hosp Med Ctr / Dept of Pediatrics

Chicago, Illinois, United States

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Cook County Hosp

Chicago, Illinois, United States

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Chicago Children's Memorial Hosp

Chicago, Illinois, United States

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Univ of Chicago Children's Hosp

Chicago, Illinois, United States

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Tulane Univ / Charity Hosp of New Orleans

New Orleans, Louisiana, United States

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Tulane Univ Hosp of New Orleans

New Orleans, Louisiana, United States

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Johns Hopkins Hosp - Pediatric

Baltimore, Maryland, United States

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Children's Hosp of Boston

Boston, Massachusetts, United States

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Baystate Med Ctr of Springfield

Springfield, Massachusetts, United States

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Univ of Massachusetts Med School

Worcester, Massachusetts, United States

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Univ of Mississippi Med Ctr

Jackson, Mississippi, United States

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UMDNJ - Robert Wood Johnson Med School / Pediatrics

New Brunswick, New Jersey, United States

Site Status

Univ of Medicine & Dentistry of New Jersey / Univ Hosp

Newark, New Jersey, United States

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Saint Joseph's Hosp and Med Ctr/UMDNJ - New Jersey Med Schl

Newark, New Jersey, United States

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Children's Hosp at Albany Med Ctr

Albany, New York, United States

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North Shore Univ Hosp

Great Neck, New York, United States

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Schneider Children's Hosp

New Hyde Park, New York, United States

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Bellevue Hosp / New York Univ Med Ctr

New York, New York, United States

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Harlem Hosp Ctr

New York, New York, United States

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Univ of Rochester Med Ctr

Rochester, New York, United States

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State Univ of New York at Stony Brook

Stony Brook, New York, United States

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Bronx Lebanon Hosp Ctr

The Bronx, New York, United States

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Montefiore Med Ctr Adolescent AIDS Program

The Bronx, New York, United States

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Duke Univ Med Ctr

Durham, North Carolina, United States

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Children's Hosp of Philadelphia

Philadelphia, Pennsylvania, United States

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Med Univ of South Carolina

Charleston, South Carolina, United States

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Texas Children's Hosp / Baylor Univ

Houston, Texas, United States

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Children's Hospital & Medical Center / Seattle ACTU

Seattle, Washington, United States

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Ramon Ruiz Arnau Univ Hosp / Pediatrics

Bayamón, , Puerto Rico

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Univ of Puerto Rico / Univ Children's Hosp AIDS

San Juan, , Puerto Rico

Site Status

San Juan City Hosp

San Juan, , Puerto Rico

Site Status

Countries

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United States Puerto Rico

References

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Shearer WT, Rosenblatt HM, Gelman RS, Oyomopito R, Plaeger S, Stiehm ER, Wara DW, Douglas SD, Luzuriaga K, McFarland EJ, Yogev R, Rathore MH, Levy W, Graham BL, Spector SA; Pediatric AIDS Clinical Trials Group. Lymphocyte subsets in healthy children from birth through 18 years of age: the Pediatric AIDS Clinical Trials Group P1009 study. J Allergy Clin Immunol. 2003 Nov;112(5):973-80. doi: 10.1016/j.jaci.2003.07.003.

Reference Type RESULT
PMID: 14610491 (View on PubMed)

Other Identifiers

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PACTG P1009

Identifier Type: -

Identifier Source: secondary_id

ACTG P1009

Identifier Type: -

Identifier Source: org_study_id