Antifolate Effectiveness in Arthritis

NCT ID: NCT00000395

Last Updated: 2016-12-16

Study Results

Results pending

The study team has not published outcome measurements, participant flow, or safety data for this trial yet. Check back later for updates.

Basic Information

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Recruitment Status

COMPLETED

Clinical Phase

PHASE2

Total Enrollment

40 participants

Study Classification

INTERVENTIONAL

Study Start Date

1996-09-30

Study Completion Date

2002-08-31

Brief Summary

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This study looks at how the arthritis drug methotrexate works in low doses to treat rheumatoid arthritis. (High doses of methotrexate are used to treat some types of cancer.) Methotrexate blocks the action of the B-vitamin known as folic acid. We are studying the biochemical reactions affected by this vitamin because we think that blocking many of these reactions may be necessary for methotrexate to work in treating rheumatoid arthritis. Through these studies, we hope to gain a better understanding of how this drug and related drugs work as treatments for arthritis.

Detailed Description

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Low-dose methotrexate therapy suppresses autoimmune arthritis in human and animal models. We hypothesize that the effect of methotrexate in the treatment of rheumatoid arthritis is due to the inhibition of aminoimidazole-carboxamide ribotide transformylase, a folate-dependent enzyme that catalyzes the last step in the de novo biosynthesis of inosine monophosphate. The resulting accumulation of aminoimidazole carboxamide riboside inhibits adenosine deaminase, therefore interfering with normal adenosine metabolism. It is well known that children with adenosine deaminase deficiency have severe combined immunodeficiency syndrome. This suggests that adenosine deaminase activity is key to immune competence and is associated with the mechanism of efficacy in methotrexate therapy of rheumatoid arthritis.

Several studies indicate that supplemental folinic acid (5-formyltetrahydrofolate) used in large doses during low-dose methotrexate therapy for rheumatoid arthritis causes a flare in joint inflammation. However, supplemental folic acid (pteroylglutamic acid) does not lessen the efficacy of the therapy. We further hypothesize that if methotrexate efficacy is driven by aminoimidazole carboxamide ribotide transformylase inhibition, folic acid supplementation should not alter urinary levels of aminoimidazole carboxamide, adenosine, and deoxyadenosine, while folinic acid supplementation should prevent the accumulation of these compounds.

We will test our hypotheses both in people with rheumatoid arthritis and in Lewis rat adjuvant arthritis. Our objectives include: (1) determining if the dose level of methotrexate that is clinically optimal in the treatment of Lewis rat adjuvant arthritis interferes with normal adenosine metabolism; (2) determining the effectiveness of drugs that interfere with adenosine metabolism (deoxycoformycin, aminoimidazole carboxamide, and aminoimidazole carboxamide with a suboptimal dose of methotrexate) in Lewis rat adjuvant arthritis; and (3) determining whether supplemental folic acid and folinic acid during methotrexate therapy normalize adenosine metabolism in patients with rheumatoid arthritis. The information we obtain will enhance the understanding of the biochemical action of antifolates/antimetabolites that are effective in the treatment of human and animal arthritis.

Conditions

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Rheumatoid Arthritis Adjuvant Arthritis

Keywords

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Rheumatoid arthritis Lewis rat adjuvant arthritis Antifolate efficacy Autoimmunity Adenosine metabolism Methotrexate therapy Skeletal disorder Dietary supplement Antiarthritic agent Folic acid

Study Design

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Allocation Method

RANDOMIZED

Intervention Model

CROSSOVER

Primary Study Purpose

DIAGNOSTIC

Blinding Strategy

DOUBLE

Participants Investigators

Study Groups

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Group 1 - Folinic acid

Subjects receiving Methotrexate for 6 weeks and 5 mg of Folinic acid daily for 1 week.

Group Type EXPERIMENTAL

Methotrexate

Intervention Type DRUG

Folinic acid

Intervention Type DIETARY_SUPPLEMENT

Group 2: Folic acid

Subjects receiving Methotrexate for 6 weeks and 5 mg of Folic acid daily for 1 week.

Group Type EXPERIMENTAL

Methotrexate

Intervention Type DRUG

Folic acid

Intervention Type DIETARY_SUPPLEMENT

Interventions

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Methotrexate

Intervention Type DRUG

Folinic acid

Intervention Type DIETARY_SUPPLEMENT

Folic acid

Intervention Type DIETARY_SUPPLEMENT

Eligibility Criteria

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Inclusion Criteria

* Individuals starting methotrexate for rheumatoid arthritis.
* Study subjects should not currently be taking folic acid-containing vitamins.

Exclusion Criteria

* Cancer, renal, or liver disease.
* Previous use of methotrexate within the past 6 months or current use of folic acid-containing supplements.
Minimum Eligible Age

18 Years

Maximum Eligible Age

85 Years

Eligible Sex

ALL

Accepts Healthy Volunteers

No

Sponsors

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National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS)

NIH

Sponsor Role collaborator

Office of Dietary Supplements (ODS)

NIH

Sponsor Role collaborator

University of Alabama at Birmingham

OTHER

Sponsor Role lead

Responsible Party

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Responsibility Role SPONSOR

Principal Investigators

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Sarah L. Morgan, M.D., R.D.

Role: PRINCIPAL_INVESTIGATOR

University of Alabama Department of Nutrition Sciences

Locations

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The University of Alabama at Birmingham

Birmingham, Alabama, United States

Site Status

Countries

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United States

References

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Morgan SL, Baggott JE, Vaughn WH, Young PK, Austin JV, Krumdieck CL, Alarcon GS. The effect of folic acid supplementation on the toxicity of low-dose methotrexate in patients with rheumatoid arthritis. Arthritis Rheum. 1990 Jan;33(1):9-18. doi: 10.1002/art.1780330102.

Reference Type BACKGROUND
PMID: 2405864 (View on PubMed)

Morgan SL, Baggott JE, Refsum H, Ueland PM. Homocysteine levels in patients with rheumatoid arthritis treated with low-dose methotrexate. Clin Pharmacol Ther. 1991 Nov;50(5 Pt 1):547-56. doi: 10.1038/clpt.1991.180.

Reference Type BACKGROUND
PMID: 1934868 (View on PubMed)

Morgan SL, Hine RJ, Vaughn WH, Brown A. Dietary intake and circulating vitamin levels of rheumatoid arthritis patients treated with methotrexate. Arthritis Care Res. 1993 Mar;6(1):4-10. doi: 10.1002/art.1790060103.

Reference Type BACKGROUND
PMID: 8443257 (View on PubMed)

Morgan SL, Baggott JE, Vaughn WH, Austin JS, Veitch TA, Lee JY, Koopman WJ, Krumdieck CL, Alarcon GS. Supplementation with folic acid during methotrexate therapy for rheumatoid arthritis. A double-blind, placebo-controlled trial. Ann Intern Med. 1994 Dec 1;121(11):833-41. doi: 10.7326/0003-4819-121-11-199412010-00002.

Reference Type BACKGROUND
PMID: 7978695 (View on PubMed)

Morgan SL, Anderson AM, Hood SM, Matthews PA, Lee JY, Alarcon GS. Nutrient intake patterns, body mass index, and vitamin levels in patients with rheumatoid arthritis. Arthritis Care Res. 1997 Feb;10(1):9-17. doi: 10.1002/art.1790100103.

Reference Type BACKGROUND
PMID: 9313385 (View on PubMed)

Morgan SL, Baggott JE, Alarcon GS. Methotrexate in rheumatoid arthritis: folate supplementation should always be given. BioDrugs. 1997 Sep;8(3):164-75. doi: 10.2165/00063030-199708030-00002.

Reference Type BACKGROUND
PMID: 18020507 (View on PubMed)

Morgan SL, Baggott JE, Lee JY, Alarcon GS. Folic acid supplementation prevents deficient blood folate levels and hyperhomocysteinemia during longterm, low dose methotrexate therapy for rheumatoid arthritis: implications for cardiovascular disease prevention. J Rheumatol. 1998 Mar;25(3):441-6.

Reference Type BACKGROUND
PMID: 9517760 (View on PubMed)

Baggott JE, Morgan SL, Koopman WJ. The effect of methotrexate and 7-hydroxymethotrexate on rat adjuvant arthritis and on urinary aminoimidazole carboxamide excretion. Arthritis Rheum. 1998 Aug;41(8):1407-10. doi: 10.1002/1529-0131(199808)41:83.0.CO;2-H.

Reference Type BACKGROUND
PMID: 9704638 (View on PubMed)

Strand V, Morgan SL, Baggott JE, Alarcon GS. Folic acid supplementation and methotrexate efficacy: comment on articles by Schiff, Emery et al, and others. Arthritis Rheum. 2000 Nov;43(11):2615-6. doi: 10.1002/1529-0131(200011)43:113.0.CO;2-A. No abstract available.

Reference Type BACKGROUND
PMID: 11083294 (View on PubMed)

Alarcon GS, Morgan SL. Guidelines for folate supplementation in rheumatoid arthritis patients treated with methotrexate: comment on the guidelines for monitoring drug therapy. Arthritis Rheum. 1997 Feb;40(2):391; author reply 391-2. doi: 10.1002/art.1780400229. No abstract available.

Reference Type BACKGROUND
PMID: 9041955 (View on PubMed)

Morgan SL, Alarcon GS, Moreland L. Improved methotrexate patient information. Arthritis Rheum. 1995 Jun;38(6):874-5. doi: 10.1002/art.1780380633. No abstract available.

Reference Type BACKGROUND
PMID: 7779139 (View on PubMed)

Morgan Sl, Alarcon GS, Krumdieck CL. Folic acid supplementation during methotrexate therapy: it makes sense. J Rheumatol. 1993 Jun;20(6):929-30. No abstract available.

Reference Type BACKGROUND
PMID: 8350325 (View on PubMed)

Morgan SL, Oster RA, Lee JY, Alarcon GS, Baggott JE. The effect of folic acid and folinic acid supplements on purine metabolism in methotrexate-treated rheumatoid arthritis. Arthritis Rheum. 2004 Oct;50(10):3104-11. doi: 10.1002/art.20516.

Reference Type RESULT
PMID: 15476202 (View on PubMed)

Other Identifiers

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R29AR042674

Identifier Type: NIH

Identifier Source: org_study_id

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R29AR042674

Identifier Type: NIH

Identifier Source: secondary_id

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NIAMS-035

Identifier Type: -

Identifier Source: secondary_id